IN-VIVO PROTEIN-DNA INTERACTIONS AT THE C-JUN PROMOTER IN QUIESCENT AND SERUM-STIMULATED FIBROBLASTS

被引:27
作者
ROZEK, D [1 ]
PFEIFER, GP [1 ]
机构
[1] BECKMAN RES INST CITY HOPE,DEPT BIOL,DUARTE,CA 91010
关键词
TRANSCRIPTION FACTORS; SERUM STIMULATION; EARLY RESPONSE GENE; IN VIVO FOOTPRINTING; CELL CYCLE;
D O I
10.1002/jcb.240570313
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
c-Jun is an important component in the regulation of cell proliferation. As a member of the early response gene family, c-jun is induced within minutes in the presence of mitogenic agents such as serum growth factors. Using in vivo footprinting, we have analyzed protein-DNA interactions at the c-jun promoter in human fibroblasts subjected to growth arrest and serum stimulation. We located seven footprints upstream of the transcription initiation site. Protein-DNA interactions were detected at two AP-1-like sequences, a CCAAT box, an SP-1 sequence, an NF-jun sequence, a putative RSRF (related to serum response factor) binding site, and a sequence bound by an unknown factor. All of these binding sites were occupied in serum-starved cells, and no additional protein-DNA interactions were detected upon serum stimulation. Evidence from this study supports a model in which expression of the c-jun gene is mediated by phosphorylation events taking place on the transactivation domains of promoter-bound transcriptional activators. (C) 1995 Wiley-Liss, Inc.
引用
收藏
页码:479 / 487
页数:9
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