A COMPARISON OF METHODS FOR QUANTITATING THE FERMENTATION OF WOOD DERIVED SUGARS TO SOLVENTS

被引：10

作者：

SCHWALD, W ^{[1
]}

SADDLER, JN ^{[1
]}

机构：

[1] FORINTEK CANADA CORP,DEPT BIOTECHNOL & CHEM,OTTAWA K1G 3Z5,ONTARIO,CANADA

来源：

ENZYME AND MICROBIAL TECHNOLOGY | 1988年 / 10卷 / 01期

关键词：

BIOTECHNOLOGY - CALORIMETRY - CELLULOSE - Degradation - CHROMATOGRAPHIC ANALYSIS - SOLVENTS - Analysis;

D O I：

10.1016/0141-0229(88)90096-8

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

A comparison was made between high performance liquid chromatography (h. p. l. c. ) and colorimeteric and gas chromatography (g. c. ) methods for quantitating the conversion of wood derived sugars to a variety of solvents. Steam-treated aspenwood, which was previously pretreated to selectively remove the lignin or hemicellulose components, was subsequently extracted into a xylan rich water-soluble fraction and a cellulose rich water-insoluble fraction. The hydrolyzed fractions were used as the substrates for growth and solvent production by either Klebsiella pneumoniae, Clostridium acetobutylicum and Candida shehatae. Sugar utilization and solvent production could be determined by a single h. p. l. c. analysis and gave values that were comparable to those determined by g. c and colorimetric methods.

引用

页码：37 / 41

页数：5

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