UP-REGULATION OF THE INTERLEUKIN-6-SIGNAL TRANSDUCING PROTEIN (GP130) BY INTERLEUKIN-6 AND DEXAMETHASONE IN HEPG2 CELLS

被引：68

作者：

SCHOOLTINK, H ^{[1
]}

SCHMITZVANDELEUR, H ^{[1
]}

HEINRICH, PC ^{[1
]}

ROSEJOHN, S ^{[1
]}

机构：

[1] RHEIN WESTFAL TH AACHEN,INST BIOCHEM,PAUWELSSTR 30,W-5100 AACHEN,GERMANY

来源：

FEBS LETTERS | 1992年 / 297卷 / 03期

关键词：

HEPATIC IL-6-RECEPTOR; GP130; INTERLEUKIN-6; GLUCOCORTICOID; ACUTE-PHASE RESPONSE; SIGNAL TRANSDUCTION;

D O I：

10.1016/0014-5793(92)80552-R

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The hepatic IL-6-receptor is composed of an 80 kDa IL-6-binding protein and a 130 kDa polypeptide (gp 130) believed to be involved in signal transduction. Previous experiments have shown that the 80 kDa IL-6-receptor is up-regulated by glucocorticoids, but not by IL-6. Here we demonstrate that IL-6 together with the synthetic glucocorticoid dexamethasone induces the expression of mRNA for gp130 approximately 5-fold in HepG2 cells. The induction was dose- and time-dependent. Dexamethasone alone, interferon-gamma, IL-1-alpha and IL-1-beta had no effect. A possible role for the regulation of the IL-6-signal transducing protein gp130 in various inflammatory states is proposed.

引用

页码：263 / 265

页数：3

共 26 条

[1] RECOMBINANT HUMAN B-CELL STIMULATORY FACTOR-II (BSF-2/IFN-BETA2) REGULATES BETA-FIBRINOGEN AND ALBUMIN MESSENGER-RNA LEVELS IN FAO-9 CELLS [J].

ANDUS, T ;

GEIGER, T ;

HIRANO, T ;

NORTHOFF, H ;

GANTER, U ;

BAUER, J ;

KISHIMOTO, T ;

HEINRICH, PC .

FEBS LETTERS, 1987, 221 (01) :18-22

[2]

[Anonymous], 1989, Ann N Y Acad Sci, V557, P1

[3] SINGLE-STEP PURIFICATION AND STRUCTURAL CHARACTERIZATION OF HUMAN INTERLEUKIN-6 PRODUCED IN ESCHERICHIA-COLI FROM A T7 RNA-POLYMERASE EXPRESSION VECTOR [J].

ARCONE, R ;

PUCCI, P ;

ZAPPACOSTA, F ;

FONTAINE, V ;

MALORNI, A ;

MARINO, G ;

CILIBERTO, G .

EUROPEAN JOURNAL OF BIOCHEMISTRY, 1991, 198 (03) :541-547

[4]

BAUMANN H, 1987, J IMMUNOL, V139, P4122

[5]

BAUMANN M, 1990, J BIOL CHEM, V265, P19853

[6] HEMATOPOIETIC RECEPTORS AND HELICAL CYTOKINES [J].

BAZAN, JF .

IMMUNOLOGY TODAY, 1990, 11 (10) :350-354

[7] STRUCTURAL DESIGN AND MOLECULAR EVOLUTION OF A CYTOKINE RECEPTOR SUPERFAMILY [J].

BAZAN, JF .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1990, 87 (18) :6934-6938

[8] A NOVEL FAMILY OF GROWTH-FACTOR RECEPTORS - A COMMON BINDING DOMAIN IN THE GROWTH-HORMONE, PROLACTIN, ERYTHROPOIETIN AND IL-6 RECEPTORS, AND THE P75 IL-2 RECEPTOR BETA-CHAIN [J].

BAZAN, JF .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1989, 164 (02) :788-795

[9] INTERLEUKIN-6 IS THE MAJOR REGULATOR OF ACUTE PHASE PROTEIN-SYNTHESIS IN ADULT HUMAN HEPATOCYTES [J].

CASTELL, JV ;

GOMEZLECHON, MJ ;

DAVID, M ;

ANDUS, T ;

GEIGER, T ;

TRULLENQUE, R ;

FABRA, R ;

HEINRICH, PC .

FEBS LETTERS, 1989, 242 (02) :237-239

[10] A TECHNIQUE FOR RADIOLABELING DNA RESTRICTION ENDONUCLEASE FRAGMENTS TO HIGH SPECIFIC ACTIVITY [J].

FEINBERG, AP ;

VOGELSTEIN, B .

ANALYTICAL BIOCHEMISTRY, 1983, 132 (01) :6-13

← 1 2 3 →