THE PROMOTER OF THE GENE ITR1 FROM BARLEY CONFERS A DIFFERENT TISSUE-SPECIFICITY IN TRANSGENIC TOBACCO

被引：20

作者：

DIAZ, I ^{[1
]}

ROYO, J ^{[1
]}

OCONNOR, A ^{[1
]}

CARBONERO, P ^{[1
]}

机构：

[1] UNIV POLITECN MADRID, ETSI AGRON, DEPT BIOTECHNOL, DEPT BIOQUIM & BIOL MOLEC, E-28040 MADRID, SPAIN

来源：

MOLECULAR AND GENERAL GENETICS | 1995年 / 248卷 / 05期

关键词：

BARLEY TRYPSIN INHIBITOR; PROMOTER; TISSUE-SPECIFIC EXPRESSION; TRANSGENIC TOBACCO;

D O I：

10.1007/BF02423455

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Tissue-specific expression of the gene coding for trypsin inhibitor BTI-CMe in barley (Itr1) occurs during the first half of endosperm development. In transgenic tobacco, the Itr1 promoter drives expression of the beta-glucuronidase reporter gene not only in developing endosperm but also in embryo, cotyledons and the meristematic intercotyledonary zone of germinating seedlings. A promoter fragment extending 343 bp upstream of the translation initiation ATG codon was sufficient for full transgene expression, whereas, the proximal 83 bp segment of the promoter was inactive. Possible reasons for the differences in expression patterns are discussed.

引用

页码：592 / 598

页数：7

共 38 条

[21] ENDOSPERM ORIGIN, DEVELOPMENT, AND FUNCTION [J].