RENATURATION OF DENATURED-LAMBDA REPRESSOR REQUIRES HEAT-SHOCK PROTEINS

被引：126

作者：

GAITANARIS, GA

PAPAVASSILIOU, AG

RUBOCK, P

SILVERSTEIN, SJ

GOTTESMAN, ME

机构：

[1] COLUMBIA UNIV COLL PHYS & SURG,INTEGRATED PROGRAM CELLULAR MOLEC & BIOPHYS STUDIES,NEW YORK,NY 10032

[2] COLUMBIA UNIV COLL PHYS & SURG,DEPT MICROBIOL,NEW YORK,NY 10032

[3] COLUMBIA UNIV COLL PHYS & SURG,DEPT BIOCHEM & MOLEC BIOPHYS,NEW YORK,NY 10032

来源：

CELL | 1990年 / 61卷 / 06期

基金：

美国国家卫生研究院;

关键词：

D O I：

10.1016/0092-8674(90)90066-N

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The temperature-sensitive bacteriophage λc1857 repressor protein rapidly renatures after thermal inactivation. E. coli mutants in the heat shock protein genes dnaK, dnaJ, and grpE do not efficiently reactivate heat-denatured repressor. Our results suggest that protein refolding is promoted by heat shock proteins and that such a process is the basis of the homeostatic role played by these proteins in the heat shock response. © 1990.

引用

收藏

页码：1013 / 1020

页数：8

相关论文

共 56 条

[11] CONSENSUS SEQUENCE FOR ESCHERICHIA-COLI HEAT-SHOCK GENE PROMOTERS [J].

COWING, DW ;

BARDWELL, JCA ;

CRAIG, EA ;

WOOLFORD, C ;

HENDRIX, RW ;

GROSS, CA .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1985, 82 (09) :2679-2683

[12]

DAMBLY-CHAUDIERE C, 1983, Journal of Molecular and Applied Genetics, V2, P45

[13] A SUBFAMILY OF STRESS PROTEINS FACILITATES TRANSLOCATION OF SECRETORY AND MITOCHONDRIAL PRECURSOR POLYPEPTIDES [J].

DESHAIES, RJ ;

KOCH, BD ;

WERNERWASHBURNE, M ;

CRAIG, EA ;

SCHEKMAN, R .

NATURE, 1988, 332 (6167) :800-805

[14] REGULATION OF REPRESSOR EXPRESSION IN LAMBDA [J].

EISEN, H ;

BRACHET, P ;

PEREIRAD.L ;

JACOB, F .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1970, 66 (03) :855-&

[15] EQUILIBRIA AND KINETICS OF LAC REPRESSOR-OPERATOR INTERACTIONS BY POLYACRYLAMIDE-GEL ELECTROPHORESIS [J].

FRIED, M ;

CROTHERS, DM .

NUCLEIC ACIDS RESEARCH, 1981, 9 (23) :6505-6525

[16] INDICATIONS OF AN INVOLVEMENT OF HEAT-SHOCK PROTEINS IN RESTORATION OF REPRESSION OF TEMPERATIVE-INDUCIBLE LAMBDA-PROPHAGE [J].

FUERST, CR .

VIROLOGY, 1987, 159 (01) :183-186

[17] A GEL-ELECTROPHORESIS METHOD FOR QUANTIFYING THE BINDING OF PROTEINS TO SPECIFIC DNA REGIONS - APPLICATION TO COMPONENTS OF THE ESCHERICHIA-COLI LACTOSE OPERON REGULATORY SYSTEM [J].

GARNER, MM ;

REVZIN, A .

NUCLEIC ACIDS RESEARCH, 1981, 9 (13) :3047-3060

[18] HOST PARTICIPATION IN BACTERIOPHAGE-LAMBDA HEAD ASSEMBLY [J].

GEORGOPOULOS, CP ;

HENDRIX, RW ;

CASJENS, SR ;

KAISER, AD .

JOURNAL OF MOLECULAR BIOLOGY, 1973, 76 (01) :45-+

[19] ROLE OF HOST-CELL IN BACTERIOPHAGE MORPHOGENESIS - EFFECTS OF A BACTERIAL MUTATION ON T4 HEAD ASSEMBLY [J].

GEORGOPOULOS, CP ;

KAISER, AD ;

WOOD, WB .

NATURE-NEW BIOLOGY, 1972, 239 (89) :38-+

[20] EXPRESSION OF WILD-TYPE AND MUTANT FORMS OF INFLUENZA HEMAGGLUTININ - THE ROLE OF FOLDING IN INTRACELLULAR-TRANSPORT [J].

GETHING, MJ ;

MCCAMMON, K ;

SAMBROOK, J .

CELL, 1986, 46 (06) :939-950

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