FIBER OPTIC SENSOR FOR CA2+ BASED ON AN INDUCED CHANGE IN THE CONFORMATION OF THE PROTEIN CALMODULIN

被引:23
作者
BLAIR, TL
YANG, ST
SMITHPALMER, T
BACHAS, LG
机构
[1] UNIV KENTUCKY,DEPT CHEM,LEXINGTON,KY 40506
[2] UNIV SOUTH,DEPT CHEM,SEWANEE,TN 37375
[3] ST FRANCIS XAVIER UNIV,DEPT CHEM,ANTIGONISH B2G 1C0,NS,CANADA
关键词
D O I
10.1021/ac00074a019
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
A fiber optic sensor that exploits the natural selectivity of the Ca2+-binding protein calmodulin (CaM) is described. In this sensor, a dialysis membrane is used to entrap a fluorescein-labeled CaM (F-CaM) solution at the common end of a bifurcated fiber optic bundle. Ca2+ ions in a sample solution can diffuse through the membrane and bind to the F-CaM. Upon binding with Ca2+, CaM undergoes a conformational change that induces a change in the fluorescence of the attached fluorescein tag. This change in fluorescence can be related to the concentration of Ca2+ in the sample solution. The detection limit for the sensor is 5 x 10(-8) M Ca2+. The sensor has no interference by Mg2+ at concentrations as high as 10(-2) M.
引用
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页码:300 / 302
页数:3
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