IDENTIFICATION OF BETA-EXOTOXIN PRODUCTION, PLASMIDS ENCODING BETA-EXOTOXIN, AND A NEW EXOTOXIN IN BACILLUS-THURINGIENSIS BY USING HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY

被引：61

作者：

LEVINSON, BL ^{[1
]}

KASYAN, KJ ^{[1
]}

CHIU, SS ^{[1
]}

CURRIER, TC ^{[1
]}

GONZALEZ, JM ^{[1
]}

机构：

[1] ECOGEN INC, LANGHORNE, PA 19047 USA

来源：

JOURNAL OF BACTERIOLOGY | 1990年 / 172卷 / 06期

关键词：

D O I：

10.1128/jb.172.6.3172-3179.1990

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

An improved high-performance liquid chromatography separation was developed to detect and quantify β-exotoxin production in Bacillus thuringiensis culture supernatants. Exotoxin production was assigned to a plasmid in five strains, from three subspecies (B. thuringiensis subsp. thuringiensis serotype 1, B. thuringiensis subsp. tolworthi serotype 9, and B. thuringiensis subsp. darmstadiensis serotype 10). A new exotoxin, called type II β-exotoxin in this report, was discovered in B. thuringiensis subsp. morrisoni serotype 8ab, purified, and partially characterized. This material is more specific than type I β-exotoxin and is very active against the Colorado potato beetle, Leptinotarsa decemlineata.

引用

页码：3172 / 3179

页数：8