DETERMINATION OF 1-LYSINE IN FEEDS BY FLUOROMETRIC METHOD BASED ON 1-LYSINE OXIDASE

被引：14

作者：

HIKUMA, M ^{[1
]}

KAWARAI, M ^{[1
]}

TONOOKA, Y ^{[1
]}

HASHIMOTO, K ^{[1
]}

机构：

[1] AJINOMOTO CO INC,TOKYO,TOKYO 104,JAPAN

来源：

ANALYTICAL LETTERS | 1991年 / 24卷 / 12期

关键词：

FLUORESCENCE; 1-LYSINE; 1-LYSINE OXIDASE; HOMOVANILLIC ACID; FEED; L-LYSINE; ENZYME ELECTRODE;

D O I：

10.1080/00032719108053047

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Feeds or feed stuffs were hydrolyzed by acid to liberate l-lysine. Homovanillic acid, peroxidase and l-lysine oxidase were used to produce the fluorescence substance corresponding to l-lysine. A computerized fluorometer was used for the measurement (up to 0.7 mg/l of l-lysine in the optical cell) and the time required for the measurement was about 1 min. The reproducibility was 1.0% in CV. Recovery tests and comparative tests against the conventional method were carried out. The effects of various experimental conditions on the fluorescence response were discussed.

引用

页码：2225 / 2236

页数：12

共 9 条

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