DIAGNOSTIC APPLICATION OF MONOCLONAL-ANTIBODIES TO OUTER-MEMBRANE PROTEIN FOR RAPID DETECTION OF SALMONELLA

被引：24

作者：

KERR, S

BALL, HJ

MACKIE, DP

POLLOCK, DA

FINLAY, DA

机构：

[1] Bacteriology Department, Veterinary Sciences Division, Belfast, Stormont

来源：

JOURNAL OF APPLIED BACTERIOLOGY | 1992年 / 72卷 / 04期

关键词：

D O I：

10.1111/j.1365-2672.1992.tb01839.x

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Monoclonal antibodies produced to Salmonella enteritidis outer membrane proteins were screened against 57 Salmonella serovars and several related enterobacteria. Those detecting all Salmonella serovars and none of the related enterobacteria were used in a microtitre plate antigen capture ELISA to screen clinical samples. Sixty-one of 2100 samples yielded salmonellas after incubation for 24 h in selective media by conventional culture. Of these 58 were detected by the ELISA. Sixty-five false positives by ELISA were found to be Enterobacter spp. The results show the potential of this ELISA to eliminate a large proportion of the salmonella-negative cultures at an early stage.

引用

页码：302 / 308

页数：7

共 23 条

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[2]

BRENNER DJ, 1984, BERGEYS MANUAL SYSTE, V1, P408

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