A GENETIC-LINKAGE MAP OF THE MOUSE USING AN EXPANDED PRODUCTION SYSTEM OF RESTRICTION LANDMARK GENOMIC SCANNING (RLGS-VER-1.8)

被引：16

作者：

OKAZAKI, Y

OKUIZUMI, H

SASAKI, N

OHSUMI, T

KUROMITSU, J

KATAOKA, H

MURAMATSU, M

IWADATE, A

HIROTA, N

KITAJIMA, M

PLASS, C

CHAPMAN, VM

HAYASHIZAKI, Y

机构：

[1] INST PHYS & CHEM RES,TSUKUBA LIFE SCI CTR,GENOME SCI LAB,TSUKUBA,IBARAKI 305,JAPAN

[2] NEW BIOTECHNOL CO LTD,DIV RES & DEV,KOCHI 780,JAPAN

[3] JAPAN SYNTHET RUBBER CO LTD,TSUKUBA RES LAB,TSUKUBA,IBARAKI 305,JAPAN

[4] ROSWELL PK CANC INST,DEPT MOLEC & CELLULAR BIOL,BUFFALO,NY 14263

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1994年 / 205卷 / 03期

关键词：

D O I：

10.1006/bbrc.1994.2895

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We have developed an expanded system (RLGS Ver.1.8) for producing RLGS patterns that result in a 16-fold increase in the number of gels produced and a 10-fold reduction in the total cost per gel. The major modifications include: 1) performing the blocking and labeling step without phenol extraction or ethanol precipitation; 2) minimizing the reaction volume and the enzyme units in each step; 3) developing a long vertical agarose disc gel electrophoresis for the Ist-dimension; and 4) developing a new apparatus for multiplex vertical 2nd-dimensional electrophoresis. RLGS Ver.1.8 was used with a new combination of restriction enzymes to identify variation for 209 loci between C57BL/6J and DBA/2J. Twenty-six BXD RI strains were analyzed and 195/209 loci were genetically mapped. These loci were mapped in one week of laboratory work by two people. This system provides an important tool for the genetic analysis of new loci in similar genetic resources. (C) 1994 Academic Press, Inc.

引用

页码：1922 / 1929

页数：8

共 14 条

[1] NUMBER OF CPG ISLANDS AND GENES IN HUMAN AND MOUSE [J].

ANTEQUERA, F ;

BIRD, A .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1993, 90 (24) :11995-11999

[2] A GENOMIC SCANNING METHOD FOR HIGHER ORGANISMS USING RESTRICTION SITES AS LANDMARKS [J].

HATADA, I ;

HAYASHIZAKI, Y ;

HIROTSUNE, S ;

KOMATSUBARA, H ;

MUKAI, T .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1991, 88 (21) :9523-9527

[3] RESTRICTION LANDMARK GENOMIC SCANNING METHOD AND ITS VARIOUS APPLICATIONS [J].

HAYASHIZAKI, Y ;

HIROTSUNE, S ;

OKAZAKI, Y ;

HATADA, I ;

SHIBATA, H ;

KAWAI, J ;

HIROSE, K ;

WATANABE, S ;

FUSHIKI, S ;

WADA, S ;

SUGIMOTO, T ;

KOBAYAKAWA, K ;

KAWARA, T ;

KATSUKI, M ;

SHIBUYA, T ;

MUKAI, T .

ELECTROPHORESIS, 1993, 14 (04) :251-258

[4] IDENTIFICATION OF AN IMPRINTED U2AF BINDING-PROTEIN RELATED SEQUENCE ON MOUSE CHROMOSOME-11 USING THE RLGS METHOD [J].

HAYASHIZAKI, Y ;

SHIBATA, H ;

HIROTSUNE, S ;

SUGINO, H ;

OKAZAKI, Y ;

SASAKI, N ;

HIROSE, K ;

IMOTO, H ;

OKUIZUMI, H ;

MURAMATSU, M ;

KOMATSUBARA, H ;

SHIROISHI, T ;

MORIWAKI, K ;

KATSUKI, M ;

HATANO, N ;

SASAKI, H ;

UEDA, T ;

MISE, N ;

TAKAGI, N ;

PLASS, C ;

CHAPMAN, VM .

NATURE GENETICS, 1994, 6 (01) :33-40

[5]

HAYASHIZAKI Y, 1994, IN PRESS ENCY MOL BI

[6]

HAYASHIZAKI Y, 1994, GENETICS, V138, P1

[7]

HIROSE K, UNPUB

[8]

HIROTSUNE S, 1992, CANCER RES, V52, P3642

[9] MOLECULAR-CLONING OF POLYMORPHIC MARKERS ON RLGS GEL USING THE SPOT TARGET CLONING METHOD [J].

HIROTSUNE, S ;

SHIBATA, H ;

OKAZAKI, Y ;

SUGINO, H ;

IMOTO, H ;

SASAKI, N ;

HIROSE, K ;

OKUIZUMI, H ;

MURAMATSU, M ;

PLASS, C ;

CHAPMAN, VM ;

TAMATSUKURI, S ;

MIYAMOTO, C ;

FURUICHI, Y ;

HAYASHIZAKI, Y .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1993, 194 (03) :1406-1412

[10]

IMOTO H, 1994, IN PRESS DNA RES

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