DETECTION OF SELENIUM-CONTAINING BIOLOGICAL CONSTITUENTS BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY PLASMA SOURCE-MASS SPECTROMETRY

被引：54

作者：

SUZUKI, KT ^{[1
]}

ITOH, M ^{[1
]}

OHMICHI, M ^{[1
]}

机构：

[1] CHIBA CITY INST HLTH,CHIBA 261,JAPAN

来源：

JOURNAL OF CHROMATOGRAPHY B-BIOMEDICAL APPLICATIONS | 1995年 / 666卷 / 01期

关键词：

D O I：

10.1016/0378-4347(94)00561-I

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Mass spectrometry with inductively coupled argon plasma excitation (ICP-MS) was used as a multi-element specific detection method for HPLC for the speciation of selenium (Se) in biological samples. Se-containing biological constituents were separated on a size-exclusion column and were detected Se-specifically at mit 78 and 82 for natural abundance Se-78 and Se-82, respectively. Se peaks not identical with known authentic samples were detected. Diets with different Se contents induced changes in the distributions of Se-containing constituents more in urine, kidney and liver samples than in plasma and red blood cells samples. The results indicate that HPLC-ICP-MS is a specific and sensitive means for the speciation of Se-containing biological constituents.

引用

页码：13 / 19

页数：7

共 30 条

[1]

BEHNE D, 1988, BIOCHIM BIOPHYS ACTA, V996, P12

[2]

BOCK A, 1991, TRENDS BIOCHEM SCI, V15, P463

[3]

BRICKER TM, SPECTROSCOPY ACROSS

[4] SOME CHARACTERISTICS OF SE-75-P, A SELENOPROTEIN FOUND IN RAT-LIVER AND PLASMA, AND COMPARISON OF IT WITH SELENOGLUTATHIONE PEROXIDASE [J].