THE CELL-CYCLE-REGULATED BUDDING YEAST GENE DBF2, ENCODING A PUTATIVE PROTEIN-KINASE, HAS A HOMOLOG THAT IS NOT UNDER CELL-CYCLE CONTROL

被引:58
作者
TOYN, JH
ARAKI, H
SUGINO, A
JOHNSTON, LH
机构
[1] NATL INST MED RES,CELL PROPAGAT LAB,RIDGEWAY,MILL HILL,LONDON NW7 1AA,ENGLAND
[2] NIEHS,MOLEC GENET LAB,RES TRIANGLE PK,NC 27709
关键词
RECOMBINANT DNA; GENE REGULATION; GENETIC MAPPING; MESSENGER RNA EXPRESSION; SACCHAROMYCES-CEREVISIAE; SEQUENCE HOMOLOGY;
D O I
10.1016/0378-1119(91)90465-N
中图分类号
Q3 [遗传学];
学科分类号
071007 ; 090102 ;
摘要
The budding yeast cell-cycle gene, DBF2, encoding a putative protein kinase, was shown to have a homologue, designated DBF20. This gene was cloned, sequenced, and confirmed to be highly homologous to DBF2, with over 80% identity in the 490 most C-terminal amino acid residues. Either gene could be deleted by itself, but deletion of both genes simultaneously was lethal, indicating that they are redundant for at least one vital function in yeast. In contrast to the DBF2 mRNA, which is expressed under cell-cycle control at or near START [Johnston et al., Mol. Cell. Biol. 10 (1990) 1358-1366], the DBF20 mRNA is expressed at a low level and not under cell-cycle control. Assuming there is no translational control, the differential expression of the mRNAs would result in a cell-cycle fluctuation of the relative levels of the gene products, which may constitute a novel form of regulation.
引用
收藏
页码:63 / 70
页数:8
相关论文
共 37 条
[1]   CHARACTERIZATION OF THE CDC7 GENE-PRODUCT OF SACCHAROMYCES-CEREVISIAE AS A PROTEIN-KINASE NEEDED FOR THE INITIATION OF MITOTIC DNA-SYNTHESIS [J].
BAHMAN, M ;
BUCK, V ;
WHITE, A ;
ROSAMOND, J .
BIOCHIMICA ET BIOPHYSICA ACTA, 1988, 951 (2-3) :335-343
[2]   SACCHAROMYCES-CEREVISIAE CDC9, A STRUCTURAL GENE FOR YEAST DNA-LIGASE WHICH COMPLEMENTS SCHIZOSACCHAROMYCES-POMBE CDC17 [J].
BARKER, DG ;
JOHNSTON, LH .
EUROPEAN JOURNAL OF BIOCHEMISTRY, 1983, 134 (02) :315-319
[3]   SEPARATION OF CHROMOSOMAL DNA-MOLECULES FROM YEAST BY ORTHOGONAL-FIELD-ALTERNATION GEL-ELECTROPHORESIS [J].
CARLE, GF ;
OLSON, MV .
NUCLEIC ACIDS RESEARCH, 1984, 12 (14) :5647-5664
[4]   2 DIFFERENTIALLY REGULATED MESSENGER-RNAS WITH DIFFERENT 5' ENDS ENCODE SECRETED AND INTRACELLULAR FORMS OF YEAST INVERTASE [J].
CARLSON, M ;
BOTSTEIN, D .
CELL, 1982, 28 (01) :145-154
[5]   GENOMIC SEQUENCING [J].
CHURCH, GM ;
GILBERT, W .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES, 1984, 81 (07) :1991-1995
[6]   TYROSINE PHOSPHORYLATION OF THE FISSION YEAST CDC2+ PROTEIN-KINASE REGULATES ENTRY INTO MITOSIS [J].
GOULD, KL ;
NURSE, P .
NATURE, 1989, 342 (6245) :39-45
[7]   THE PROTEIN-KINASE FAMILY - CONSERVED FEATURES AND DEDUCED PHYLOGENY OF THE CATALYTIC DOMAINS [J].
HANKS, SK ;
QUINN, AM ;
HUNTER, T .
SCIENCE, 1988, 241 (4861) :42-52
[8]   3 ADDITIONAL GENES REQUIRED FOR DEOXYRIBONUCLEIC ACID SYNTHESIS IN SACCHAROMYCES-CEREVISIAE [J].
HARTWELL, LH .
JOURNAL OF BACTERIOLOGY, 1973, 115 (03) :966-974
[9]   SACCHAROMYCES-CEREVISIAE CELL-CYCLE [J].
HARTWELL, LH .
BACTERIOLOGICAL REVIEWS, 1974, 38 (02) :164-198
[10]   ISOLATION OF YEAST HISTONE GENES H2A AND H2B [J].
HEREFORD, L ;
FAHRNER, K ;
WOOLFORD, J ;
ROSBASH, M ;
KABACK, DB .
CELL, 1979, 18 (04) :1261-1271