IDENTIFICATION OF TISSUE PROTEINS BY AMINO-ACID-ANALYSIS AFTER PURIFICATION BY 2-DIMENSIONAL ELECTROPHORESIS

被引：44

作者：

JUNGBLUT, P ^{[1
]}

DZIONARA, M ^{[1
]}

KLOSE, J ^{[1
]}

WITTMANNLEIBOLD, B ^{[1
]}

机构：

[1] MAX PLANCK INST MOLEC GENET, W-1000 BERLIN 33, GERMANY

来源：

JOURNAL OF PROTEIN CHEMISTRY | 1992年 / 11卷 / 06期

关键词：

2-DIMENSIONAL ELECTROPHORESIS; BLOTTING; AMINO ACID ANALYSIS; DATABASES; IDENTIFICATION; PROTEINS;

D O I：

10.1007/BF01024960

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Mouse brain proteins were separated by two-dimensional electrophoresis (2-DE). The proteins of a section of the 2-DE pattern were blotted onto hydrophobic membranes and 43 of them were excised and hydrolyzed by liquid-phase hydrolysis. The amino acid composition of these proteins was determined by orthophthaldialdehyde precolumn derivatization and compared with the compositions of known proteins stored in the NBRF sequence database. An identification program named ASA was developed for this purpose. The ASA program includes correction and weighting factors, data reduction by molecular weight windows, and exclusion or inclusion of certain organisms as desired. As a control, eight test proteins and five well-known proteins from mouse brain, all separated by 2-DE, were correctly identified by the program. Out of the 43 brain proteins selected, 19 were identified with high confidence.

引用

页码：603 / 612

页数：10

共 30 条

[11] DETERMINATION OF AMINO-ACIDS WITH 9-FLUORENYLMETHYL CHLOROFORMATE AND REVERSED-PHASE HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY [J].

EINARSSON, S ;

JOSEFSSON, B ;

LAGERKVIST, S .

JOURNAL OF CHROMATOGRAPHY, 1983, 282 (DEC) :609-618

[12]

EINARSSON S, 1983, J CHROMATOGR, V28, P92

[13] MICROSEQUENCING OF PROTEINS ELECTROTRANSFERRED ONTO IMMOBILIZING MATRICES FROM POLYACRYLAMIDE-GEL ELECTROPHORESIS - APPLICATION TO AN INSOLUBLE PROTEIN [J].

HIRANO, H ;

WATANABE, T .

ELECTROPHORESIS, 1990, 11 (07) :573-580

[14] BLOTTING EFFICIENCY INVESTIGATED BY USING 2-DIMENSIONAL ELECTROPHORESIS, HYDROPHOBIC MEMBRANES AND PROTEINS FROM DIFFERENT SOURCES [J].

JUNGBLUT, P ;

ECKERSKORN, C ;

LOTTSPEICH, F ;

KLOSE, J .

ELECTROPHORESIS, 1990, 11 (07) :581-588

[15]

JUNGBLUT P, 1992, UNPUB

[16]

JUNGBLUT PR, 1989, BIOL CHEM H-S, V370, P775

[17] SEQUENCING OF PROTEINS FROM TWO-DIMENSIONAL GELS BY USING INSITU DIGESTION AND TRANSFER OF PEPTIDES TO POLYVINYLIDENE DIFLUORIDE MEMBRANES - APPLICATION TO PROTEINS ASSOCIATED WITH SENSITIZATION IN APLYSIA [J].

KENNEDY, TE ;

GAWINOWICZ, MA ;

BARZILAI, A ;

KANDEL, ER ;

SWEATT, JD .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1988, 85 (18) :7008-7012

[18]

KLOSE J, 1975, HUMANGENETIK, V26, P231

[19]

KLOSE J, 1983, MODERN METHODS PROTE, P49

[20] CLEAVAGE OF STRUCTURAL PROTEINS DURING ASSEMBLY OF HEAD OF BACTERIOPHAGE-T4 [J].

LAEMMLI, UK .

NATURE, 1970, 227 (5259) :680-+

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