FUNCTIONAL EXPRESSION OF AN ALPHA-ANTIINSECT SCORPION NEUROTOXIN IN INSECT CELLS AND LEPIDOPTEROUS LARVAE

被引：53

作者：

CHEJANOVSKY, N

ZILBERBERG, N

RIVKIN, H

ZLOTKIN, E

GUREVITZ, M

机构：

[1] TEL AVIV UNIV,DEPT BOT,IL-69978 TEL AVIV,ISRAEL

[2] HEBREW UNIV JERUSALEM,INST LIFE SCI,IL-91904 JERUSALEM,ISRAEL

来源：

FEBS LETTERS | 1995年 / 376卷 / 03期

关键词：

LQH-ALPHA-IT (LEIURUS QUINQUESTRIATUS HEBRAEUS); ANTIINSECT TOXIN; BACULOVIRUS; INSECT CELLS; LEPIDOPTEROUS LARVAE;

D O I：

10.1016/0014-5793(95)01263-4

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The Leiurus quinquestriatus hebraeus alpha anti-insect toxin (Lqh alpha IT) cDNA was engineered into the Autographa californica Nuclear Polyhedrosis Virus (AcNPV) genome. Insect cells infected with the recombinant virus secreted a functional Lqh alpha IT polypeptide. Spodoptera littoralis and Heliothis armigera larvae injected with recombinant budded virus, showed typical intoxication symptoms. This recombinant virus showed enhanced insecticidal potency against H. armigera larvae compared with mild type AcNPV. The present expression system will facilitate: (1) the future elucidation of structural elements involved in its prominent anti-insect toxicity; and (2) the future design of genetically modified alpha toxins with improved anti-insect selectivity.

引用

页码：181 / 184

页数：4

共 14 条

[1] BACULOVIRUS EXPRESSION OF ALKALINE-PHOSPHATASE AS A REPORTER GENE FOR EVALUATION OF PRODUCTION, GLYCOSYLATION AND SECRETION [J].