PREPARATION AND INITIAL CHARACTERIZATION OF CRYSTALS OF THE PHOTOPROTEIN AEQUORIN FROM AEQUOREA-VICTORIA

被引:11
作者
HANNICK, LI
PRASHER, DC
SCHULTZ, LW
DESCHAMPS, JR
WARD, KB
机构
[1] GEOCENTERS INC,FT WASHINGTON,MD 20744
[2] WOODS HOLE OCEANOG INST,WOODS HOLE,MA 02543
[3] CORNELL UNIV,DEPT CHEM,ITHACA,NY 14853
来源
PROTEINS-STRUCTURE FUNCTION AND GENETICS | 1993年 / 15卷 / 01期
关键词
AEQUORIN; PHOTOPROTEIN; CRYSTALLIZATION; BIOLUMINESCENCE; X-RAY DIFFRACTION; METAL-BINDING;
D O I
10.1002/prot.340150113
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Crystals of recombinant aequorin, the photoprotein from the jellyfish Aequorea victoria, have been grown from solutions containing sodium phosphate. The crystals grow as thin plates which diffract to beyond 2.2 angstrom resolution. The crystals are orthorhombic, space group P2(1)2(1)2(1); the axes are a = 89.1(1), b = 88.4(1), and c = 52.7(1) angstrom. The asymmetric unit contains two molecules. Crystals exposed to calcium ion solutions emit a steady glow and slowly deteriorate, confirming that the crystals consist of a charged, competent photoprotein. This represents the first successful preparation of single crystals of a photoprotein suitable for diffraction analysis.
引用
收藏
页码:103 / 107
页数:5
相关论文
共 24 条
[1]   MEASUREMENT OF CA-2+ CONCENTRATIONS IN LIVING CELLS [J].
BLINKS, JR ;
WIER, WG ;
HESS, P ;
PRENDERGAST, FG .
PROGRESS IN BIOPHYSICS & MOLECULAR BIOLOGY, 1982, 40 (1-2) :1-114
[2]  
BLINKS JR, 1976, PHARMACOL REV, V28, P1
[3]   EXTENSION OF MOLECULAR REPLACEMENT - A NEW SEARCH STRATEGY BASED ON PATTERSON CORRELATION REFINEMENT [J].
BRUNGER, AT .
ACTA CRYSTALLOGRAPHICA SECTION A, 1990, 46 :46-57
[4]   AMINO-ACID SEQUENCE OF THE CALCIUM-DEPENDENT PHOTOPROTEIN AEQUORIN [J].
CHARBONNEAU, H ;
WALSH, KA ;
MCCANN, RO ;
PRENDERGAST, FG ;
CORMIER, MJ ;
VANAMAN, TC .
BIOCHEMISTRY, 1985, 24 (24) :6762-6771
[5]  
COOK WJ, 1991, J BIOL CHEM, V266, P652
[6]  
Cormier M.J., 1978, P75
[7]  
Dubois R, 1885, CR SOC BIOL, V37, P559
[8]   MERLOT, AN INTEGRATED PACKAGE OF COMPUTER-PROGRAMS FOR THE DETERMINATION OF CRYSTAL-STRUCTURES BY MOLECULAR REPLACEMENT [J].
FITZGERALD, PMD .
JOURNAL OF APPLIED CRYSTALLOGRAPHY, 1988, 21 (03) :273-278
[9]  
HARVEY E. N., 1952, BIOLUMINESCENCE
[10]  
Harvey EN, 1921, BIOL BULL, V51, P89