PREPARATION AND INITIAL CHARACTERIZATION OF CRYSTALS OF THE PHOTOPROTEIN AEQUORIN FROM AEQUOREA-VICTORIA

被引：11

作者：

HANNICK, LI

PRASHER, DC

SCHULTZ, LW

DESCHAMPS, JR

WARD, KB

机构：

[1] GEOCENTERS INC,FT WASHINGTON,MD 20744

[2] WOODS HOLE OCEANOG INST,WOODS HOLE,MA 02543

[3] CORNELL UNIV,DEPT CHEM,ITHACA,NY 14853

来源：

PROTEINS-STRUCTURE FUNCTION AND GENETICS | 1993年 / 15卷 / 01期

关键词：

AEQUORIN; PHOTOPROTEIN; CRYSTALLIZATION; BIOLUMINESCENCE; X-RAY DIFFRACTION; METAL-BINDING;

D O I：

10.1002/prot.340150113

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Crystals of recombinant aequorin, the photoprotein from the jellyfish Aequorea victoria, have been grown from solutions containing sodium phosphate. The crystals grow as thin plates which diffract to beyond 2.2 angstrom resolution. The crystals are orthorhombic, space group P2(1)2(1)2(1); the axes are a = 89.1(1), b = 88.4(1), and c = 52.7(1) angstrom. The asymmetric unit contains two molecules. Crystals exposed to calcium ion solutions emit a steady glow and slowly deteriorate, confirming that the crystals consist of a charged, competent photoprotein. This represents the first successful preparation of single crystals of a photoprotein suitable for diffraction analysis.

引用

页码：103 / 107

页数：5

共 24 条

[21] EXTRACTION PURIFICATION AND PROPERTIES OF BIOLUMINESCENCE SYSTEM OF EUPHAUSID SHRIMP MEGANYCTIPHANES NORVEGICA [J].