HIGH-DENSITY CDNA FILTER ANALYSIS - A NOVEL-APPROACH FOR LARGE-SCALE, QUANTITATIVE-ANALYSIS OF GENE-EXPRESSION

被引:94
作者
ZHAO, ND
HASHIDA, H
TAKAHASHI, N
MISUMI, Y
SAKAKI, Y
机构
[1] UNIV TOKYO,CTR HUMAN GENOME,INST MED SCI,MINATO KU,TOKYO 108,JAPAN
[2] UNIV TOKYO,INST BRAIN RES,DEPT NEUROL,BUNKYO KU,TOKYO 113,JAPAN
[3] FUKUOKA UNIV,SCH MED,DEPT BIOCHEM,JONAN KU,FUKUOKA 81401,JAPAN
关键词
HYBRIDIZATION; GENE EXPRESSION PROFILES; ADULT BRAIN; FETAL BRAIN;
D O I
10.1016/0378-1119(95)00023-Y
中图分类号
Q3 [遗传学];
学科分类号
071007 ; 090102 ;
摘要
In order to analyze the expression profiles of a large number of genes in the tissues (or cells) of interest, and to identify the genes preferentially expressed in the tissues, we have developed a large-scale gene expression analysis system. It is based on the hybridization of the mRNAs from the tissues with a high-density cDNA filter followed by the quantitative measurement of the amount of the hybridized mRNA on each cDNA spot. By employing a high-performance bioimaging analyzer, the system allowed us to compare the expression profiles of thousands of genes (cDNAs) simultaneously with a sensitivity comparable to conventional Northern blotting analysis. By this system (called high-density cDNA filter analysis or HDCFA), the expression profiles of 2505 cloned human brain cDNAs (genes) were monitored. Through the comparison of the expression profiles of these cDNAs in the adult brain, fetal brain and adult liver, about one half of these brain cDNAs (1239 clones) were identified as the candidates which were expressed preferentially in the brain. Among these, 408 and 288 clones were found to be preferentially expressed in the adult and fetal brain, respectively. The results have shown that the system may be widely applicable for analysis of the gene expression profiles of various tissues on a large scale.
引用
收藏
页码:207 / 213
页数:7
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