LISTERIA-MONOCYTOGENES MUTANTS LACKING PHOSPHATIDYLINOSITOL-SPECIFIC PHOSPHOLIPASE-C ARE AVIRULENT

被引：160

作者：

CAMILLI, A ^{[1
]}

GOLDFINE, H ^{[1
]}

PORTNOY, DA ^{[1
]}

机构：

[1] UNIV PENN,SCH MED,DEPT MICROBIOL,209 JOHNSON PAVILION,PHILADELPHIA,PA 19104

来源：

JOURNAL OF EXPERIMENTAL MEDICINE | 1991年 / 173卷 / 03期

关键词：

D O I：

10.1084/jem.173.3.751

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

A number of bacterial species secrete phosphatidylinositol-specific phospholipase C (PI-PLC). In this report, we show that the facultative intracellular bacterial pathogen, Listeria monocytogenes, contains a gene, plcA, predicting a polypeptide with 31% amino acid identity to a Bacillus thuringiensis PI-PLC. Accordingly, L. monocytogenes secretes PI-PLC activity, while a mutant with a transposon insertion in plcA lacks detectable PI-PLC activity. In addition, expression of plcA in B. subtilis resulted in secretion of PI-PLC activity. The L. monocytogenes PI-PLC-defective mutant was three logs less virulent for mice and failed to grow in host tissues. The mutant was also defective for in vitro growth in mouse peritoneal macrophages. These results strongly suggest that PI-PLC is an essential determinant of L. monocytogenes pathogenesis. Whether the PI-PLC acts on a bacterial or host substrate remains to be determined.

引用

页码：751 / 754

页数：4

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