CRYSTAL-STRUCTURE OF ESCHERICHIA-COLI QOR QUINONE OXIDOREDUCTASE COMPLEXED WITH NADPH

被引：98

作者：

THORN, JM ^{[1
]}

BARTON, JD ^{[1
]}

DIXON, NE ^{[1
]}

OLLIS, DL ^{[1
]}

EDWARDS, KJ ^{[1
]}

机构：

[1] AUSTRALIAN NATL UNIV,RES SCH CHEM,CTR MOLEC STRUCT & FUNCT,CANBERRA,ACT 0200,AUSTRALIA

来源：

JOURNAL OF MOLECULAR BIOLOGY | 1995年 / 249卷 / 04期

关键词：

QUINONE OXIDOREDUCTASE; ALCOHOL DEHYDROGENASE; ZETA-CRYSTALLIN; CRYSTAL STRUCTURE; NADPH;

D O I：

10.1006/jmbi.1995.0337

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The crystal structure of the homodimer of quinone oxidoreductase from Escherichia coli has been determined using the multiple isomorphous replacement method at 2.2 Angstrom resolution and refined to an R-factor of 14.1% The crystallographic asymmetric unit contains one functional dimer with the two subunits being related by a non-crystallographic 2-fold symmetry axis. The model consists of two polypeptide chains (residues 2 through 327), one NADPH molecule and one sulphate anion per subunit, and 432 water molecules. Each subunit consists of two domains: a catalytic domain and a nucleotide-binding domain with the NADPH co-factor bound in the cleft between domains. Quinone oxidoreductase has an unusual nucleotide-binding fingerprint motif consisting of the sequence AXXGXXG. The overall structure of quinone oxidoreductase shows strong structural homology to that of horse liver alcohol dehydrogenase.

引用

页码：785 / 799

页数：15

共 33 条

[1] BELLOMO G, 1987, CHEM SCRIPTA, V27A, P117
[2] PROTEIN DATA BANK - COMPUTER-BASED ARCHIVAL FILE FOR MACROMOLECULAR STRUCTURES
BERNSTEIN, FC
KOETZLE, TF
WILLIAMS, GJB
MEYER, EF
BRICE, MD
RODGERS, JR
KENNARD, O
SHIMANOUCHI, T
TASUMI, M
[J]. JOURNAL OF MOLECULAR BIOLOGY, 1977, 112 (03) : 535 - 542
[3] EYE LENS ZETA-CRYSTALLIN RELATIONSHIPS TO THE FAMILY OF LONG-CHAIN ALCOHOL POLYOL DEHYDROGENASES - PROTEIN TRIMMING AND CONSERVATION OF STABLE PARTS
BORRAS, T
PERSSON, B
JORNVALL, H
[J]. BIOCHEMISTRY, 1989, 28 (15) : 6133 - 6139
[4] BRUNGER AT, 1992, X PLOR V3 1 MANUAL S
[5] CRYSTALLIZATION AND PRELIMINARY-X-RAY DIFFRACTION STUDIES ON A SOLUBLE ESCHERICHIA-COLI QUINONE OXIDOREDUCTASE
EDWARDS, KJ
THORN, JM
DANIHER, JA
DIXON, NE
OLLIS, DL
[J]. JOURNAL OF MOLECULAR BIOLOGY, 1994, 240 (05) : 501 - 503
[6] MOLECULAR ASPECTS OF FUNCTIONAL DIFFERENCES BETWEEN ALCOHOL AND SORBITOL DEHYDROGENASES
EKLUND, H
HORJALES, E
JORNVALL, H
BRANDEN, CI
JEFFERY, J
[J]. BIOCHEMISTRY, 1985, 24 (27) : 8005 - 8012
[7] 3-DIMENSIONAL STRUCTURE OF HORSE LIVER ALCOHOL-DEHYDROGENASE AT 2.4 A RESOLUTION
EKLUND, H
NORDSTROM, B
ZEPPEZAUER, E
SODERLUND, G
OHLSSON, I
BOIWE, T
SODERBERG, BO
TAPIA, O
BRANDEN, CI
AKESON, A
[J]. JOURNAL OF MOLECULAR BIOLOGY, 1976, 102 (01) : 27 - &
[8] CRYSTALLOGRAPHIC INVESTIGATIONS OF NICOTINAMIDE ADENINE-DINUCLEOTIDE BINDING TO HORSE LIVER ALCOHOL-DEHYDROGENASE
EKLUND, H
SAMAMA, JP
JONES, TA
[J]. BIOCHEMISTRY, 1984, 23 (25) : 5982 - 5996
[9] Eklund H., 1987, BIOL MACROMOLECULES, V3
[10] ERNSTER L, 1987, CHEM SCRIPTA, V27A, P1

← 1 2 3 4 →