REMEASUREMENT AT HIGH RESOLVING POWER IN FOURIER-TRANSFORM ION-CYCLOTRON RESONANCE MASS-SPECTROMETRY

The Fourier transform ion cyclotron resonance mass spectrometry remeasurement experiment is demonstrated and evaluated under high resolution conditions. Signal-to-noise enhancement is observed for isotopically resolved bovine insulin peaks at a resolution of similar to 31,000 (full width at half height). The experiment is sensitive to space-charge effects and resultant changes in scan-to-scan signal-to-noise and resolution, Coulombic repulsion in the ion cloud during the high resolution remeasurement experiment can cause the cyclotron frequency to shift through the duration of the experiment, which results in broadened peak shapes when individual remeasurement spectra are coadded. By either reducing the number of ions in the cell or allowing the ion cloud to diffuse during the lifetime of the experiment, high resolution remeasurement spectra can be coadded without peak broadening or degradation of signal-to-noise ratio.