DETECTION OF HUMAN HEPATITIS-A VIRUS IN ENVIRONMENTAL WATER BY AN ANTIGEN-CAPTURE POLYMERASE CHAIN-REACTION METHOD

被引：22

作者：

PREVOT, J ^{[1
]}

DUBROU, S ^{[1
]}

MARECHAL, J ^{[1
]}

机构：

[1] LAB HYG PARIS,F-75013 PARIS,FRANCE

来源：

WATER SCIENCE AND TECHNOLOGY | 1993年 / 27卷 / 3-4期

关键词：

HEPATITIS-A VIRUS; IMMUNOAFFINITY CAPTURE; HEMI-NESTED ENZYMATIC AMPLIFICATION; HYBRIDIZATION; ENVIRONMENTAL WATER;

D O I：

10.2166/wst.1993.0351

中图分类号：

X [环境科学、安全科学];

学科分类号：

08 ; 0830 ;

摘要：

To detect hepatitis A virus (HAV) in environmental water samples, sensitive and specific methods are needed. An hemi-nested enzymatic amplification procedure was developed. When it was associated with an immuno-capture (IC) step, specificity and sensitivity were increased. The presence of a specific DNA fragment of 318 bp was detected by the analysis of the electrophoresis of the PCR products and confirmed on the autoradiogram of the Southern blot of the gel, using a labeled oligoprobe PA1 selected in a very highly conserved region of the genome coding for the capsid protein VPI. The IC/PCR detected less than one infectious unit of virus in 75 mul sample. Eleven Seine River samples and two drinking water specimens were monitored by this assay. The IC/PCR brings a significant contribution for increasing our knowledge of the incidence of HAV on public health.

引用

页码：227 / 233

页数：7

共 15 条

[1] CYTOMEGALOVIRUS DNA DETECTION OF AN IMMEDIATE EARLY PROTEIN GENE WITH NESTED PRIMER OLIGONUCLEOTIDES [J].