STRUCTURE OF AN ANTIIDIOTYPIC FAB AGAINST FELINE PERITONITIS VIRUS-NEUTRALIZING ANTIBODY AND A COMPARISON WITH THE COMPLEXED FAB

被引：15

作者：

BAN, N

ESCOBAR, C

HASEL, KW

DAY, J

GREENWOOD, A

MCPHERSON, A

机构：

[1] UNIV CALIF RIVERSIDE,DEPT BIOCHEM,RIVERSIDE,CA 92521

[2] IMMUNOPHARMACEUT INC,SAN DIEGO,CA 92127

来源：

FASEB JOURNAL | 1995年 / 9卷 / 01期

关键词：

CRYSTAL STRUCTURE; INTERNAL IMAGE; CONFORMATIONAL CHANGE; INDUCED FIT;

D O I：

10.1096/fasebj.9.1.7821749

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The crystal structure of anti-idiotopic Fab 409.5.3, made against an E2 specific feline infectious peritonitis virus-neutralizing antibody 730.1.4, has been determined in its free form, at 2.9 Angstrom resolution by molecular replacement. This antibody, used as an immunogen, elicits the production of anti-anti-idiotypic antibodies that in turn neutralize the virus. The structure of the uncomplexed Fab was refined using constrained-restrained least squares minimization and simulated annealing in combination with conjugate gradient techniques to a crystallographic R of 0.22 based on 16,482 unique reflections between 20.0 and 2.9 Angstrom. The free antiidiotypic Fab shows, when compared to its complexed form, a 5 degrees rotation of its variable light with respect to its variable heavy domain and rearrangement of complementarity determining region loops, which permits optimization of the stereocomplementarity between interacting molecules. This finding supports the induced fit hypothesis for antibody antigen interaction.

引用

页码：107 / 114

页数：8

共 58 条

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