ENZYME SENSOR FOR L-LACTATE WITH A CHITOSAN-MERCURY FILM ELECTRODE

被引：28

作者：

HIKIMA, S ^{[1
]}

KAKIZAKI, T ^{[1
]}

TAGA, M ^{[1
]}

HASEBE, K ^{[1
]}

机构：

[1] HOKKAIDO UNIV,FAC SCI,DEPT CHEM,SAPPORO,HOKKAIDO 060,JAPAN

来源：

FRESENIUS JOURNAL OF ANALYTICAL CHEMISTRY | 1993年 / 345卷 / 8-9期

关键词：

D O I：

10.1007/BF00325809

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

An amperometric enzyme sensor composed of a mercury film electrode and an enzyme-immobilized chitosan membrane is developed. This biosensor is based on both a mercury film electrode detecting the consumption of dissolved dioxygen following enzymatic reaction, and a chitosan membrane. The latter provides an excellent permselectivity and excludes electroactive interferents. The detection range of this biosensor was 1.0 x 10(-5)-3.0 x 10(-4) mol/l and the relative standard deviation, R.S.D. at 5.0 x 10(-5) mol/l was 1.4% (n = 3). This biosensor was applied to the direct determination of L-lactate in human serum without pretreatment.

引用

页码：607 / 609

页数：3

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