REAL-TIME OBSERVATION OF LOW-TEMPERATURE PROTEIN MOTIONS

被引：61

作者：

LEESON, DT ^{[1
]}

WIERSMA, DA ^{[1
]}

机构：

[1] UNIV GRONINGEN,CTR MAT SCI,9747 AG GRONINGEN,NETHERLANDS

来源：

PHYSICAL REVIEW LETTERS | 1995年 / 74卷 / 11期

关键词：

D O I：

10.1103/PhysRevLett.74.2138

中图分类号：

O4 [物理学];

学科分类号：

0702 ;

摘要：

Optical methods were used to study the internal motions of myoglobin and cytochrome c. The experiments show that these proteins exhibit conformational fluctuations at temperatures as low as 2 K. The distribution of fluctuation rates can be measured in real time and turns out to be very sharp. The temperature dependence of the structural relaxation of myoglobin follows a simple Arrhenius law. The results are in agreement with existing models for protein dynamics. © 1995 The American Physical Society.

引用

页码：2138 / 2141

页数：4

共 22 条

[1] ANOMALOUS LOW-TEMPERATURE THERMAL PROPERTIES OF GLASSES AND SPIN GLASSES [J].

ANDERSON, PW ;

HALPERIN, BI ;

VARMA, CM .

PHILOSOPHICAL MAGAZINE, 1972, 25 (01) :1-&

[2] PROTEIN STATES AND PROTEIN QUAKES [J].

ANSARI, A ;

BERENDZEN, J ;

BOWNE, SF ;

FRAUENFELDER, H ;

IBEN, IET ;

SAUKE, TB ;

SHYAMSUNDER, E ;

YOUNG, RD .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1985, 82 (15) :5000-5004

[3] TIME SCALES AND OPTICAL DEPHASING MEASUREMENTS - INVESTIGATION OF DYNAMICS IN COMPLEX-SYSTEMS [J].

BAI, YS ;

FAYER, MD .

PHYSICAL REVIEW B, 1989, 39 (15) :11066-11084

[4] MULTIPLE CONFORMATIONAL STATES OF PROTEINS - A MOLECULAR-DYNAMICS ANALYSIS OF MYOGLOBIN [J].

ELBER, R ;

KARPLUS, M .

SCIENCE, 1987, 235 (4786) :318-321

[5] THE ENERGY LANDSCAPES AND MOTIONS OF PROTEINS [J].

FRAUENFELDER, H ;

SLIGAR, SG ;

WOLYNES, PG .

SCIENCE, 1991, 254 (5038) :1598-1603

[6] TEMPERATURE-DEPENDENT X-RAY-DIFFRACTION AS A PROBE OF PROTEIN STRUCTURAL DYNAMICS [J].

FRAUENFELDER, H ;

PETSKO, GA ;

TSERNOGLOU, D .

NATURE, 1979, 280 (5723) :558-563

[7] A COMPARATIVE PRESSURE TUNING HOLE-BURNING STUDY OF PROTOPORPHYRIN-IX IN MYOGLOBIN AND IN A GLASSY HOST [J].

GAFERT, J ;

FRIEDRICH, J ;

PARAK, F .

JOURNAL OF CHEMICAL PHYSICS, 1993, 99 (04) :2478-2486

[8] SPECTRAL-DIFFUSION DECAY IN ECHO EXPERIMENTS [J].

HU, P ;

WALKER, LR .

PHYSICAL REVIEW B, 1978, 18 (03) :1300-1305

[9] GLASSY BEHAVIOR OF A PROTEIN [J].

IBEN, IET ;

BRAUNSTEIN, D ;

DOSTER, W ;

FRAUENFELDER, H ;

HONG, MK ;

JOHNSON, JB ;

LUCK, S ;

ORMOS, P ;

SCHULTE, A ;

STEINBACH, PJ ;

XIE, AH ;

YOUNG, RD .

PHYSICAL REVIEW LETTERS, 1989, 62 (16) :1916-1919

[10] HOLE-BURNING SPECTROSCOPY AND RELAXATION DYNAMICS OF AMORPHOUS SOLIDS AT LOW-TEMPERATURES [J].

JANKOWIAK, R ;

SMALL, GJ .

SCIENCE, 1987, 237 (4815) :618-625

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