DNA PLOIDY IN CELL-NUCLEI FROM PARAFFIN-EMBEDDED MATERIAL - COMPARISON OF RESULTS FROM 2 LABORATORIES

被引：18

作者：

FOSSA, SD

BERNER, A

WAEHRE, H

HEIDEN, T

JUUL, MEH

VANDENOUDEN, D

PETTERSEN, EO

WANG, N

TRIBUKAIT, B

机构：

[1] NORWEGIAN RADIUM HOSP,DEPT SURG ONCOL,OSLO 3,NORWAY

[2] NORWEGIAN RADIUM HOSP,DEPT PATHOL,OSLO 3,NORWAY

[3] KAROLINSKA INST,INST MED RADIOBIOL,S-10401 STOCKHOLM 60,SWEDEN

[4] INST CANC RES,DEPT TISSUE CULTURE,OSLO,NORWAY

[5] ACAD HOSP ROTTERDAM,ROTTERDAM,NETHERLANDS

[6] ERASMUS UNIV,DEPT UROL,3000 DR ROTTERDAM,NETHERLANDS

来源：

CYTOMETRY | 1992年 / 13卷 / 04期

关键词：

DNA PLOIDY; PARAFFIN-EMBEDDED MATERIAL; INTERLABORATORY VARIABILITY; PROSTATE CANCER;

D O I：

10.1002/cyto.990130410

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

In 49 pairs of contiguous sections from paraffin-embedded prostatic cancer tissue, the DNA indices (DIs) were determined by flow cytometry (FCM) at 2 different laboratories. In 3 of 45 pairs of evaluable nuclear suspensions, DIs of 1.1 (DNA aneuploid) were found at Laboratory 1, whereas all 3 tumours were classified as DNA diploid at Laboratory 2. In the remaining 42 specimens, the correlation between the DIs was excellent, though the application of strictly defined DNA ploidy ranges led to different DNA ploidy allocation in 3 cases. It is concluded that in 85-90% of the cases, reliable DIs can be obtained by FCM done in paraffin-embedded material at different laboratories. Slight technical variations and interpretation differences may lead to different ploidy allocation in 10-15% of the cases.

引用

页码：395 / 403

页数：9

共 26 条

[11] AN IMPROVED HEDLEY METHOD FOR PREPARATION OF PARAFFIN-EMBEDDED TISSUES FOR FLOW CYTOMETRIC ANALYSIS OF PLOIDY AND S-PHASE [J].