POLYESTER FILM STRIPS COATED WITH PHOTOGRAPHIC GELATIN CONTAINING IMMOBILIZED INVERTASE

被引：5

作者：

AKBULUT, U ^{[1
]}

SUNGUR, S ^{[1
]}

PEKYARDIMCI, S ^{[1
]}

机构：

[1] ANKARA UNIV,FAC SCI,DEPT CHEM,ANKARA 06100,TURKEY

来源：

JOURNAL OF MACROMOLECULAR SCIENCE-CHEMISTRY | 1991年 / A28卷

关键词：

D O I：

10.1080/00222339108054406

中图分类号：

O63 [高分子化学（高聚物）];

学科分类号：

070305 ; 080501 ; 081704 ;

摘要：

Invertase was immobilized into photographic gelatin by chemical crosslinking with formaldehyde and chromium (III) acetate. Polyester film strips were coated with invertase-gelatin mixture which increased durability during consecutive immersions into reaction media. The effect of gelatin, crosslinker and enzyme concentrations on activity were studied. Enzyme leakages from immobilized invertase film strips were controlled by washing with EDTA solutions. The activities of washing solutions were measured spectrophotometrically to determine unbounded enzyme concentration. Effect of use number on activity was also determined. Percent activity of immobilized invertase was lower than that of free enzyme. The activity however retained even after 10 washings (170 minutes) followed by 9 use in 18 weeks.

引用

页码：239 / 247

页数：9

共 19 条

[1]

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[2]

AXEN R, 1974, INSOLUBILIZED ENZYME

[3]

AYKUT G, 1983, THESIS METU ANKARA T

[4] UTILIZATION OF IMMOBILIZED ENZYMES AND IMMOBILIZED MICROBIAL CELLS [J].