A NOVEL ISOFORM OF RAT HEPATOCYTE NUCLEAR FACTOR-IV (HNF-4)

被引：51

作者：

HATA, S

TSUKAMOTO, T

OSUMI, T

机构：

[1] Faculty of Science, Himeji Institute of Technology, Hyogo

来源：

BIOCHIMICA ET BIOPHYSICA ACTA | 1992年 / 1131卷 / 02期

关键词：

HEPATOCYTE NUCLEAR FACTOR-IV; DIFFERENTIAL SPLICING; POLYMERASE CHAIN REACTION; TRANSCRIPTION FACTOR;

D O I：

10.1016/0167-4781(92)90080-J

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Based on the published nucleotide sequence for rat hepatocyte nuclear factor 4 (HNF-4; Sladek, F.M., Zhong, W., Lai, E. and Darnell, J.E., Jr. (1990) Genes Dev. 4, 2353-2365), we have cloned a cDNA by means of polymerase chain reaction amplification of reverse-transcribed RNA (RT-PCR). Our clone contained an extra segment of 30 bp, which was not found in the previously reported clone, in the coding region near the C-terminus. Further RT-PCR analysis demonstrated that both isoforms of HNF-4 mRNA, i.e., with or without the 30 nucleotide segment, occur in rat liver and kidney, presumably by differential splicing.

引用

页码：211 / 213

页数：3

共 12 条

[1] SIMILARITIES IN TRANSTHYRETIN GENE-EXPRESSION AND DIFFERENCES IN TRANSCRIPTION FACTORS - LIVER AND YOLK-SAC COMPARED TO CHOROID-PLEXUS [J].