HIGH-RESOLUTION COSMID AND P1-MAPS SPANNING THE 14-MB GENOME OF THE FISSION YEAST S-POMBE

被引：233

作者：

HOHEISEL, JD

MAIER, E

MOTT, R

MCCARTHY, L

GRIGORIEV, AV

SCHALKWYK, LC

NIZETIC, D

FRANCIS, F

LEHRACH, H

机构：

[1] Imperial Cancer Research Fund Lincoln's Inn Fields, London

来源：

CELL | 1993年 / 73卷 / 01期

关键词：

D O I：

10.1016/0092-8674(93)90164-L

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Gridded on high density filters, a P1 genomic library of 17-fold coverage and a cosmid library of 8 genome equivalents, both made from S. pombe strain 972h-, were ordered by hybridizing genetic markers and individual clones from the two libraries. Yeast artificial chromosome (YAC) clones covering the entire genome were used to subdivide the libraries, and hybridization of short oligonucleotides and DNA pools made from randomly selected cosmids provided further mapping information. Restriction digests were generated as an independent confirmation of the clone order. The high resolution clone map was aligned to the genetic map and the physical NotI and YAC maps. The usefulness of the various mapping techniques and cloning procedures could be assessed upon the different data sets.

引用

页码：109 / 120

页数：12

共 35 条

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