PREPARATION OF POLYACRYLAMIDE GEL-FILLED CAPILLARIES BY PHOTOPOLYMERIZATION FOR CAPILLARY ELECTROPHORESIS

被引：6

作者：

BRUIN, GJM ^{[1
]}

WANG, T ^{[1
]}

XU, X ^{[1
]}

KRAAK, JC ^{[1
]}

POPPE, H ^{[1
]}

机构：

[1] LAB ANALYT CHEM AMSTERDAM,NIEUWE ACHTERGRACHT 166,1018 WV AMSTERDAM,NETHERLANDS

来源：

JOURNAL OF MICROCOLUMN SEPARATIONS | 1992年 / 4卷 / 05期

关键词：

CAPILLARY ELECTROPHORESIS; POLYACRYLAMIDE GELS; PREPARATION; DIFFUSION COEFFICIENTS;

D O I：

10.1002/mcs.1220040512

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A new preparation method for polyacrylamide gels in fused silica capillaries is described. In this method use is made of riboflavin as a light sensitive initiator for the polymerization reaction. Also, results from measurements on the diffusion coefficients of four oligonucleotides in a polyacrylamide gel are reported. Furthermore, a new preparative method to purify and collect oligonucleotides is presented. Separation performance of the gels was tested with various samples of oligonucleotides.

引用

页码：439 / 448

页数：10

共 24 条

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