Expression and regulation of prostaglandin F receptor mRNA in rodent osteoblastic cells

被引：14

作者：

Nemoto, K ^{[1
]}

Bernecker, PM ^{[1
]}

Pilbeam, CC ^{[1
]}

Raisz, LG ^{[1
]}

机构：

[1] UNIV CONNECTICUT,CTR HLTH,DEPT MED,DIV ENDOCRINOL & METAB,FARMINGTON,CT 06030

来源：

PROSTAGLANDINS | 1995年 / 50卷 / 5-6期

关键词：

osteoblast; receptor; prostaglandin F; rodent; dexamethasone; fluprostenol;

D O I：

10.1016/0090-6980(95)00140-9

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The ability of prostaglandins to inhibit collagen synthesis and induce prostaglandin G/H synthase in bone cells appears to be mediated by the prostaglandin F-2 alpha receptor (FPR). We have identified FPR mRNA in the osteoblastic cell lines, Pyla from rats and MC3T3-E1 from mice, as well as in the stem cell cultures, MN-7 and mouse marrow, using reverse transcriptase-polymerase chain reaction technology (RT-PCR). Expression of FPR mRNA was increased in Pyla, MN-7 and marrow cells with prolonged culture or dexamethasone treatment and decreased after treatment with fluprostenol, a selective FPR agonist.

引用

页码：349 / 358

页数：10

共 13 条

[1]

ABRAMOVITZ M, 1994, J BIOL CHEM, V269, P2632

[2]

CHOMCZYNSKI P, 1987, ANAL BIOCHEM, V162, P156, DOI 10.1016/0003-2697(87)90021-2

[3]

COLEMAN RA, 1994, PHARMACOL REV, V46, P205

[4]

FALL PM, 1994, J BONE MINER RES, V9, P1935

[5] PROSTAGLANDIN-F2-ALPHA RECEPTOR IS COUPLED TO GQ IN CDNA-TRANSFECTED CHINESE-HAMSTER OVARY CELLS [J].

ITO, S ;

SAKAMOTO, K ;

MOCHIZUKIODA, N ;

EZASHI, T ;

MIWA, K ;

OKUDAASHITAKA, E ;

SHEVCHENKO, VI ;

KISO, Y ;

HAYAISHI, O .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1994, 200 (02) :756-762

[6]

KAWAGUCHI H, 1995, CLIN ORTHOP RELAT R, V313, P36

[7] CLONING AND EXPRESSION OF A CDNA FOR RAT PROSTAGLANDIN-F2-ALPHA RECEPTOR [J].

KITANAKA, J ;

HASIMOTO, H ;

SUGIMOTO, Y ;

NEGISHI, M ;

AINO, H ;

GOTOH, M ;

ICHIKAWA, A ;

BABA, A .

PROSTAGLANDINS, 1994, 48 (01) :31-41

[8] CLONING OF THE RAT AND HUMAN PROSTAGLANDIN-F2-ALPHA RECEPTORS AND THE EXPRESSION OF THE RAT PROSTAGLANDIN-F2-ALPHA RECEPTOR [J].

LAKE, S ;

GULLBERG, H ;

WAHLQVIST, J ;

SJOGREN, AM ;

KINHULT, A ;

LIND, P ;

HELLSTROMLINDAHL, E ;

STJERNSCHANTZ, J .

FEBS LETTERS, 1994, 355 (03) :317-325

[9] INVIVO DESENSITIZATION OF A HIGH-AFFINITY PGF2-ALPHA RECEPTOR IN THE OVINE CORPUS-LUTEUM [J].

LAMSA, JC ;

CUSHMAN, RA ;

NAY, MG ;

MCCRACKEN, JA .

PROSTAGLANDINS & OTHER LIPID MEDIATORS, 1992, 43 (02) :165-179

[10]

LI DY, 1995, J PHARMACOL EXP THER, V272, P15

← 1 2 →