ESTROGEN-RECEPTOR VARIANTS IN CLINICAL BREAST-CANCER

被引:247
作者
MCGUIRE, WL
CHAMNESS, GC
FUQUA, SAW
机构
[1] Division of Medical Oncology, University of Texas Health Science Center, San Antonio, TX
关键词
D O I
10.1210/mend-5-11-1571
中图分类号
R5 [内科学];
学科分类号
1002 ; 100201 ;
摘要
We have used the screening techniques of chemical mismatch cleavage, single stranded conformational polymorphism, and gel retardation to discover a number of estrogen receptor RNA variants in clinical breast cancer tissues. We have found basepair insertions, transitions, and deletions as well as alternative splicing, yielding deletions of exon 3, 5, or 7. Using a yeast transactivation assay we have discovered receptors with outlaw function, including both a dominant-positive receptor, which is transcriptionally active in the absence of estrogen, and a dominant-negative receptor, which is itself transcriptionally inactive, but prevents the action of normal estrogen receptor. These variants could have clinical significance, helping to explain breast tumor behavior and patient outcome.
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页码:1571 / 1577
页数:7
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