STOICHIOMETRY OF THE CRE RECOMBINASE BOUND TO THE LOX RECOMBINING SITE

被引：49

作者：

MACK, A ^{[1
]}

SAUER, B ^{[1
]}

ABREMSKI, K ^{[1
]}

HOESS, R ^{[1
]}

机构：

[1] DUPONT MERCK PHARMACEUT CO,EXPTL STN,BOX 80328,WILMINGTON,DE 19880

来源：

NUCLEIC ACIDS RESEARCH | 1992年 / 20卷 / 17期

关键词：

D O I：

10.1093/nar/20.17.4451

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The site-specific recombinase Cre from bacteriophage P1 binds and carries out recombination at a 34 bp lox site. The lox site consists of two 13 bp inverted repeats, separated by an 8 bp spacer region. Both the palindromic nature of the site and the results of footprinting and band shift experiments suggest that a minimum of two Cre molecules bind to a lox site. We report here experiments that demonstrate the absolute stoichiometry of the Cre-lox complex to be one molecule of Cre bound per inverted repeat, or two molecules per lox site.

引用

页码：4451 / 4455

页数：5

共 15 条

[1]

ABREMSKI K, 1984, J BIOL CHEM, V259, P1509

[2]

CRAIG NL, 1988, ANNU REV GENET, V22, P77

[3] SPECIFIC PROTEIN DNA COMPLEXES - IMMUNODETECTION OF THE PROTEIN-COMPONENT AFTER GEL-ELECTROPHORESIS AND WESTERN BLOTTING [J].

GRANGERSCHNARR, M ;

LLOUBES, R ;

DEMURCIA, G ;

SCHNARR, M .

ANALYTICAL BIOCHEMISTRY, 1988, 174 (01) :235-238

[4]

HENDRICKSON W, 1984, J MOL BIOL, V174, P611

[5] DNA SPECIFICITY OF THE CRE RECOMBINASE RESIDES IN THE 25 KDA CARBOXYL DOMAIN OF THE PROTEIN [J].

HOESS, R ;

ABREMSKI, K ;

IRWIN, S ;

KENDALL, M ;

MACK, A .

JOURNAL OF MOLECULAR BIOLOGY, 1990, 216 (04) :873-882

[6] INTERACTION OF THE BACTERIOPHAGE-P1 RECOMBINASE CRE WITH THE RECOMBINING SITE LOXP [J].

HOESS, RH ;

ABREMSKI, K .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES, 1984, 81 (04) :1026-1029

[7]

HOESS RH, 1990, NUCLEIC ACIDS MOL BI, V4, P99

[8] GCN4, A EUKARYOTIC TRANSCRIPTIONAL ACTIVATOR PROTEIN, BINDS AS A DIMER TO TARGET DNA [J].

HOPE, IA ;

STRUHL, K .

EMBO JOURNAL, 1987, 6 (09) :2781-2784

[9] CLEAVAGE OF STRUCTURAL PROTEINS DURING ASSEMBLY OF HEAD OF BACTERIOPHAGE-T4 [J].

LAEMMLI, UK .

NATURE, 1970, 227 (5259) :680-+

[10]

Maniatis T., 1982, MOL CLONING

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