STOICHIOMETRY OF THE CRE RECOMBINASE BOUND TO THE LOX RECOMBINING SITE

被引：49

作者：

MACK, A ^{[1
]}

SAUER, B ^{[1
]}

ABREMSKI, K ^{[1
]}

HOESS, R ^{[1
]}

机构：

[1] DUPONT MERCK PHARMACEUT CO,EXPTL STN,BOX 80328,WILMINGTON,DE 19880

来源：

NUCLEIC ACIDS RESEARCH | 1992年 / 20卷 / 17期

关键词：

D O I：

10.1093/nar/20.17.4451

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The site-specific recombinase Cre from bacteriophage P1 binds and carries out recombination at a 34 bp lox site. The lox site consists of two 13 bp inverted repeats, separated by an 8 bp spacer region. Both the palindromic nature of the site and the results of footprinting and band shift experiments suggest that a minimum of two Cre molecules bind to a lox site. We report here experiments that demonstrate the absolute stoichiometry of the Cre-lox complex to be one molecule of Cre bound per inverted repeat, or two molecules per lox site.

引用

页码：4451 / 4455

页数：5

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