THE EFFECT OF EDTA AND SERUM ON ENDOGENOUS PORPHYRIN ACCUMULATION AND PHOTODYNAMIC SENSITIZATION OF HUMAN K562 LEUKEMIC-CELLS

被引：98

作者：

HANANIA, J ^{[1
]}

MALIK, Z ^{[1
]}

机构：

[1] BAR ILAN UNIV,DEPT LIFE SCI,HLTH SCI RES CTR,IL-52900 RAMAT GAN,ISRAEL

来源：

CANCER LETTERS | 1992年 / 65卷 / 02期

关键词：

PHOTOSENSITIZATION; AMINOLEVULINIC ACID; ENDOGENOUS PORPHYRIN; K562; LEUKEMIA;

D O I：

10.1016/0304-3835(92)90156-P

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

The interrelationship between the effect of serum on the induction of porphyrin synthesis, intracellular porphyrin accumulation and photodynamic sensitization of human K562 cells is described. Endogenous porphyrins, synthesized from supplemented 5-amino levulinic acid (5-ALA), were shown to accumulate in the cells, while an addition of serum triggered porphyrin translocation from the cell to the serum. In order to enhance porphyrin accumulation in the cells themselves, they were further stimulated by EDTA, which in combination with 5-ALA reduces Fe++ cellular content. The higher porphyrin cellular content under EDTA and 5-ALA induction was exploited to photoinactivate the human leukemic cells by more then 3 orders of magnitude.

引用

页码：127 / 131

页数：5