PROLACTIN AND INTERLEUKIN-2 RECEPTORS IN T-LYMPHOCYTES SIGNAL THROUGH A MGF-STAT5-LIKE TRANSCRIPTION FACTOR

被引:61
作者
GOUILLEUX, F [1 ]
MORITZ, D [1 ]
HUMAR, M [1 ]
MORIGGL, R [1 ]
BERCHTOLD, S [1 ]
GRONER, B [1 ]
机构
[1] INST EXPTL CANC RES, TUMOR BIOL CTR, D-79106 FREIBURG, GERMANY
关键词
D O I
10.1210/en.136.12.5700
中图分类号
R5 [内科学];
学科分类号
1002 ; 100201 ;
摘要
The cell surface receptors for PRL and interleukin-2 (IL-2) are structurally distinct, but share regulatory tasks in T lymphocytes. They can stimulate proliferation and activate transcription of overlapping sets of genes of T cells. PRL and IL-2 receptor activation are both linked to the Jak/Stat (signal transducer and activator of transcription) pathway. We investigated the ability of PRL and IL-2 to activate Stat proteins in different T cell lines. The DNA binding specificities, the reactivities toward Stat-specific antisera, and the mol wt of IL-2- and PRL-induced DNA-binding proteins in Nb2 and Cl96 T cell lines were investigated. A comparison with the Stat proteins induced by interferon-gamma, PRL, and IL-6 in T47D mammary tumor cells was made. We found that these parameters were indistinguishable for one of the PRL- and IL-2-induced factors. A transcription factor closely related to mammary gland factor-Stat5 is rapidly activated upon interaction of IL-2 and PRL with their respective receptors. Activation of a second protein related to Stat1 was also observed. Our results emphasize the role of PRL as a regulator of the immune response and indicate that the Stat factors mammary gland factor-Stat5 and Stat1 play a role in the regulation of gene expression during T cell development.
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页码:5700 / 5708
页数:9
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