PURIFICATION OF 14-3-3 PROTEIN AND ANALYSIS OF ISOFORMS IN CHICKEN BRAIN

被引：17

作者：

PATEL, Y ^{[1
]}

MARTIN, H ^{[1
]}

HOWELL, S ^{[1
]}

JONES, D ^{[1
]}

ROBINSON, K ^{[1
]}

AITKEN, A ^{[1
]}

机构：

[1] NATL INST MED RES,PROT STRUCT LAB,LONDON NW7 1AA,ENGLAND

来源：

BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH | 1994年 / 1222卷 / 03期

基金：

英国医学研究理事会;

关键词：

14-3-3; PROTEIN; ISOFORM-SPECIFIC ANTIBODY; PKC INHIBITOR; (BRAIN); (CHICKEN);

D O I：

10.1016/0167-4889(94)90048-5

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

14-3-3 proteins are apparently ubiquitous eukaryotic proteins that comprise a large number of isoforms. We have used specific antibodies raised against each mammalian isoform to probe for 14-3-3 isoforms in adult hen brains. The results suggest that there is a remarkable degree of similarity in primary structure (at least in the regions containing the epitopes). Reverse-phase HPLC of the purified avian 14-3-3 proteins indicates a high overall degree of similarity in sequence and levels of expression of each isoform that are remarkably similar to their mammalian counterparts.

引用

页码：405 / 409

页数：5

共 24 条

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