EFFECT OF METABOLIC-INHIBITORS AND 2ND MESSENGERS UPON NA+-H+ EXCHANGE IN THE SHEEP CARDIAC PURKINJE-FIBER

被引：49

作者：

WU, ML

VAUGHANJONES, RD

机构：

[1] University Laboratory of Physiology, Oxford

来源：

JOURNAL OF PHYSIOLOGY-LONDON | 1994年 / 478卷 / 02期

关键词：

D O I：

10.1113/jphysiol.1994.sp020251

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

1. Acid extrusion through Na+-H+ exchange was studied in the sheep cardiac Purkinje fibre (bathed in Hepes-buffered solution, nominally free of CO2-HCO3-) by examining (i) intracellular pH (pH(i)) recovery from an intracellular acid load (induced by 20 mM NH4Cl prepulse) and (ii) the rate of rise of intracellular Na+ activity (a(Na)(i)) following the ammonium prepulse (used as an estimate of apparent Na+ influx on Na+-H+ exchange). The pH(i) and a(Na)(i) were recorded using ion-selective microelectrodes. 2. The pH(i) recovery and rise of a(Na)(i) were both greatly slowed in the presence of 2-deoxyglucose (DOG; glucose-free solution), an inhibitor of glycolysis, indicating inhibition of Na+-H+ exchange.

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页码：301 / 313

页数：13

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[11] THE GIANT CARDIAC MEMBRANE PATCH METHOD - STIMULATION OF OUTWARD NA+-CA2+ EXCHANGE CURRENT BY MGATP [J].

COLLINS, A ;

SOMLYO, AV ;

HILGEMANN, DW .

JOURNAL OF PHYSIOLOGY-LONDON, 1992, 454 :27-57

[12]

COUNILLON L, 1993, SOC GEN PHY, V48, P169

[13] NA(+)-HCO3- SYMPORT IN THE SHEEP CARDIAC PURKINJE-FIBER [J].

DART, C ;

VAUGHANJONES, RD .

JOURNAL OF PHYSIOLOGY-LONDON, 1992, 451 :365-385

[14] INTERACTIONS BETWEEN THE REGULATION OF THE INTRACELLULAR PH AND SODIUM ACTIVITY OF SHEEP CARDIAC PURKINJE-FIBERS [J].

DEITMER, JW ;

ELLIS, D .

JOURNAL OF PHYSIOLOGY-LONDON, 1980, 304 (JUL) :471-488

[15] SODIUM-DEPENDENT CONTROL OF INTRACELLULAR PH IN PURKINJE-FIBERS OF SHEEP HEART [J].

ELLIS, D ;

MACLEOD, KT .

JOURNAL OF PHYSIOLOGY-LONDON, 1985, 359 (FEB) :81-105

[16] PHOSPHORYLATION OF THE C-TERMINAL DOMAIN OF THE NA+/H+ EXCHANGER BY CA2+/CALMODULIN-DEPENDENT PROTEIN KINASE-II [J].

FLIEGEL, L ;

WALSH, MP ;

SINGH, D ;

WONG, C ;

BARR, A .

BIOCHEMICAL JOURNAL, 1992, 282 :139-145

[17]

FONTERIZ RI, 1992, J PHYSL, V459, pP230

[18] ATP HYDROLYSIS ASSOCIATED WITH AN UNCOUPLED SODIUM FLUX THROUGH SODIUM-PUMP - EVIDENCE FOR ALLOSTERIC EFFECTS OF INTRACELLULAR ATP AND EXTRACELLULAR SODIUM [J].

GLYNN, IM ;

KARLISH, SJD .

JOURNAL OF PHYSIOLOGY-LONDON, 1976, 256 (02) :465-496

[19] NA-22+ FLUXES IN THYMIC LYMPHOCYTES .2. AMILORIDE-SENSITIVE NA+/H+ EXCHANGE PATHWAY - REVERSIBILITY OF TRANSPORT AND ASYMMETRY OF THE MODIFIER SITE [J].

GRINSTEIN, S ;

GOETZ, JD ;

ROTHSTEIN, A .

JOURNAL OF GENERAL PHYSIOLOGY, 1984, 84 (04) :585-600

[20] PARATHYROID-HORMONE AND DIBUTYRYL CAMP INHIBIT NA+/H+ EXCHANGE IN RENAL BRUSH-BORDER VESICLES [J].

KAHN, AM ;

DOLSON, GM ;

HISE, MK ;

BENNETT, SC ;

WEINMAN, EJ .

AMERICAN JOURNAL OF PHYSIOLOGY, 1985, 248 (02) :F212-F218

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