DETERMINATION OF ERGOSTEROL IN ORGANIC DUST BY GAS-CHROMATOGRAPHY MASS-SPECTROMETRY

被引：85

作者：

AXELSSON, BO ^{[1
]}

SARAF, A ^{[1
]}

LARSSON, L ^{[1
]}

机构：

[1] LUND UNIV, DEPT MED MICROBIOL, S-22362 LUND, SWEDEN

来源：

JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES | 1995年 / 666卷 / 01期

关键词：

D O I：

10.1016/0378-4347(94)00553-H

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A gas chromatographic-mass spectrometric method was developed for the determination of ergosterol in organic dust. Samples were hydrolyzed under alkaline conditions, and the hydrolysate was extracted, purified on a silica-gel column, and subjected to derivatization. The limit of detection of the trimethyisilyl ether derivative of ergosterol was approximately 10 pg and that of the tert. -butyldimethylsilyl ether derivative was approximately 20 pg (injected amounts). House dust contained 6-45 mu g ergosterol/g and air from a pig barn contained 0.2-0.3 ng ergosterol/liter. The proposed method can be used as a complement or alternative to microscopy and culturing for measuring fungal biomass in air-borne organic dust.

引用

页码：77 / 84

页数：8