NUCLEOTIDE-SEQUENCES OF 2 GENOMIC DNAS ENCODING PEROXIDASE OF ARABIDOPSIS-THALIANA

被引：34

作者：

INTAPRUK, C ^{[1
]}

HIGASHIMURA, N ^{[1
]}

YAMAMOTO, K ^{[1
]}

OKADA, N ^{[1
]}

SHINMYO, A ^{[1
]}

TAKANO, M ^{[1
]}

机构：

[1] OSAKA UNIV,FAC ENGN,DEPT FERMENTAT TECHNOL,SUITA,OSAKA 565,JAPAN

来源：

GENE | 1991年 / 98卷 / 02期

关键词：

RECOMBINANT DNA; HORSERADISH ENZYMES; SEQUENCE HOMOLOGIES; EXONS; INTRONS; ISOZYME;

D O I：

10.1016/0378-1119(91)90179-F

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

The peroxidase (EC 1.11.1.7)-encoding gene of Arabidopsis thaliana was screened from a genomic library using a cDNA encoding a neutral isozyme of horseradish, Armoracia rusticana, peroxidase (HRP) as a probe, and two positive clones were isolated. From the comparison with the sequences of the HRP-encoding genes, we concluded that two clones contained peroxidase-encoding genes, and they were named prxCa and prxEa. Both genes consisted of four exons and three introns; the introns had consensus nucleotides, GT and AG, at the 5' and 3' ends, respectively. The lengths of each putative exon of the prxEa gene were the same as those of the HRP-basic-isozyme-encoding gene, prxC3, and coded for 349 amino acids (aa) with a sequence homology of 89% to that encoded by prxC3. The prxCa gene was very close to the HRP-neutral-isozyme-encoding gene, prxClb, and coded for 354 aa with 91% homology to that encoded by prxClb. The aa sequence homology was 64% between the two peroxidases encoded by prxCa and prxEa.

引用

页码：237 / 241

页数：5