LINEAR CHROMOSOMAL PHYSICAL AND GENETIC-MAP OF BORRELIA-BURGDORFERI, THE LYME-DISEASE AGENT

被引:96
作者
CASJENS, S
HUANG, WM
机构
[1] Department of Cellular, Viral and Molecular Biology, University of Utah Medical Center, Salt Lake City, Utah
关键词
D O I
10.1111/j.1365-2958.1993.tb01641.x
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
A physical map of the 952 kbp chromosome of Borrelia burgdorferi Sh-2-82 has been constructed. Eighty-three intervals on the chromosome, defined by the cleavage sites of 15 restriction enzymes, are delineated. The intervals vary in size from 96 kbp to a few hundred bp, with an average size of 11.5 kbp. A striking feature of the map is its linearity; no other bacterial groups are known to have linear chromosomes. The two ends of the chromosome do not hybridize with one another, indicating that there are no large common terminal regions. The chromosome of this strain was found to be stable in culture; passage 6, 165 and 320 cultures have identical chromosomal restriction maps. We have positioned all previously known Borrelia burgdorferi chromosomal genes and several newly identified ones on this map. These include the gyrA/gyrB/dnaA/dnaN gene cluster, the rRNA gene cluster, fla, flgE, groEL (hsp60), recA, the rho/hip cluster, the dnaK (hsp70)/dnaJ/grpE cluster, the pheT/pheS cluster, and the genes which encode the potent immunogen proteins p22A, p39 and p83. Our electrophoretic analysis detects five linear and at least two circular plasmids in B. burgdorferi Sh-2-82. We have constructed a physical map of the 53 kbp linear plasmid and located the operon that encodes the two major outer surface proteins ospA and ospB on this plasmid. Because of the absence of functional genetic tools for this organism, these maps will serve as a basis for future mapping, cloning and sequencing studies of B. burgdorferi.
引用
收藏
页码:967 / 980
页数:14
相关论文
共 99 条
  • [1] PHENOTYPIC AND GENOTYPIC ANALYSIS OF BORRELIA-BURGDORFERI ISOLATES FROM VARIOUS SOURCES
    ADAM, T
    GASSMANN, GS
    RASIAH, C
    GOBEL, UB
    [J]. INFECTION AND IMMUNITY, 1991, 59 (08) : 2579 - 2585
  • [2] PHYSICAL MAP OF THE BRUCELLA-MELITENSIS 16-M CHROMOSOME
    ALLARDETSERVENT, A
    CARLESNURIT, MJ
    BOURG, G
    MICHAUX, S
    RAMUZ, M
    [J]. JOURNAL OF BACTERIOLOGY, 1991, 173 (07) : 2219 - 2224
  • [3] BORRELIA-BURGDORFERI HSP70 HOMOLOG - CHARACTERIZATION OF AN IMMUNOREACTIVE STRESS PROTEIN
    ANZOLA, J
    LUFT, BJ
    GORGONE, G
    DATTWYLER, RJ
    SODERBERG, C
    LAHESMAA, R
    PELTZ, G
    [J]. INFECTION AND IMMUNITY, 1992, 60 (09) : 3704 - 3713
  • [4] CHARACTERIZATION OF A BORRELIA-BURGDORFERI-DNAJ HOMOLOG
    ANZOLA, J
    LUFT, BJ
    GORGONE, G
    PELTZ, G
    [J]. INFECTION AND IMMUNITY, 1992, 60 (11) : 4965 - 4968
  • [5] DELINEATION OF BORRELIA-BURGDORFERI SENSU-STRICTO, BORRELIA-GARINII SP-NOV, AND GROUP VS461 ASSOCIATED WITH LYME BORRELIOSIS
    BARANTON, G
    POSTIC, D
    SAINTGIRONS, I
    BOERLIN, P
    PIFFARETTI, JC
    ASSOUS, M
    GRIMONT, PAD
    [J]. INTERNATIONAL JOURNAL OF SYSTEMATIC BACTERIOLOGY, 1992, 42 (03): : 378 - 383
  • [6] BARBOUR AG, 1984, YALE J BIOL MED, V57, P521
  • [7] THE GENES ENCODING MAJOR SURFACE-PROTEINS OF BORRELIA-BURGDORFERI ARE LOCATED ON A PLASMID
    BARBOUR, AG
    GARON, CF
    [J]. ANNALS OF THE NEW YORK ACADEMY OF SCIENCES, 1988, 539 : 144 - 153
  • [8] BIOLOGY OF BORRELIA SPECIES
    BARBOUR, AG
    HAYES, SF
    [J]. MICROBIOLOGICAL REVIEWS, 1986, 50 (04) : 381 - 400
  • [9] PLASMID ANALYSIS OF BORRELIA-BURGDORFERI, THE LYME-DISEASE AGENT
    BARBOUR, AG
    [J]. JOURNAL OF CLINICAL MICROBIOLOGY, 1988, 26 (03) : 475 - 478
  • [10] LINEAR PLASMIDS OF THE BACTERIUM BORRELIA-BURGDORFERI HAVE COVALENTLY CLOSED ENDS
    BARBOUR, AG
    GARON, CF
    [J]. SCIENCE, 1987, 237 (4813) : 409 - 411