BINDING AND MOBILITY OF ANTI-DINITROPHENYL MONOCLONAL-ANTIBODIES ON FLUID-LIKE, LANGMUIR-BLODGETT PHOSPHOLIPID MONOLAYERS CONTAINING DINITROPHENYL-CONJUGATED PHOSPHOLIPIDS

被引：23

作者：

TIMBS, MM ^{[1
]}

POGLITSCH, CL ^{[1
]}

PISARCHICK, ML ^{[1
]}

SUMNER, MT ^{[1
]}

THOMPSON, NL ^{[1
]}

机构：

[1] UNIV N CAROLINA,DEPT CHEM,CHAPEL HILL,NC 27599

来源：

BIOCHIMICA ET BIOPHYSICA ACTA | 1991年 / 1064卷 / 02期

关键词：

PLANAR MODEL MEMBRANE; CELL-SURFACE IMMUNOCHEMISTRY; TRANSLATIONAL DIFFUSION; FLUORESCENCE MICROSCOPY; FLUORESCENCE PHOTOBLEACHING RECOVERY;

D O I：

10.1016/0005-2736(91)90305-R

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The association of a fluorescently labelled anti-dinitrophenyl monoclonal antibody (ANO2) with Langmuir-Blodgett monolayers composed of three different binary mixtures of phosphatidylcholine and dinitrophenyl-conjugated phosphatidylethanolamine has been characterized. Quantitative fluorescence microscopy measurements demonstrated that measurable amounts of antibodies bound to the monolayers only at high molar fractions of dinitrophenyl-conjugated lipid (greater-than-or-equal-to 5 mol%). Fluorescence pattern photobleaching recovery measurements showed that the apparent translational diffusion coefficients and mobile fractions of a fluorescent lipid were high for all monolayer compositions and that the antibody translational mobility was measurable but slow and depended on the two-dimensional antibody density. The results demonstrate that the ANO2-binding characteristics of Langmuir-Blodgett monolayers containing dinitrophenyl-conjugated phospholipids are substantially different from those of similar model systems but that the ANO2 antibodies, when bound, display similar diffusive behavior.

引用

页码：219 / 228

页数：10

共 54 条

[1]

ANDREE HAM, 1990, J BIOL CHEM, V265, P4923

[2] MONOCLONAL-ANTIBODIES TO A NITROXIDE LIPID HAPTEN [J].