BINDING AND MOBILITY OF ANTI-DINITROPHENYL MONOCLONAL-ANTIBODIES ON FLUID-LIKE, LANGMUIR-BLODGETT PHOSPHOLIPID MONOLAYERS CONTAINING DINITROPHENYL-CONJUGATED PHOSPHOLIPIDS

被引:23
作者
TIMBS, MM [1 ]
POGLITSCH, CL [1 ]
PISARCHICK, ML [1 ]
SUMNER, MT [1 ]
THOMPSON, NL [1 ]
机构
[1] UNIV N CAROLINA,DEPT CHEM,CHAPEL HILL,NC 27599
关键词
PLANAR MODEL MEMBRANE; CELL-SURFACE IMMUNOCHEMISTRY; TRANSLATIONAL DIFFUSION; FLUORESCENCE MICROSCOPY; FLUORESCENCE PHOTOBLEACHING RECOVERY;
D O I
10.1016/0005-2736(91)90305-R
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The association of a fluorescently labelled anti-dinitrophenyl monoclonal antibody (ANO2) with Langmuir-Blodgett monolayers composed of three different binary mixtures of phosphatidylcholine and dinitrophenyl-conjugated phosphatidylethanolamine has been characterized. Quantitative fluorescence microscopy measurements demonstrated that measurable amounts of antibodies bound to the monolayers only at high molar fractions of dinitrophenyl-conjugated lipid (greater-than-or-equal-to 5 mol%). Fluorescence pattern photobleaching recovery measurements showed that the apparent translational diffusion coefficients and mobile fractions of a fluorescent lipid were high for all monolayer compositions and that the antibody translational mobility was measurable but slow and depended on the two-dimensional antibody density. The results demonstrate that the ANO2-binding characteristics of Langmuir-Blodgett monolayers containing dinitrophenyl-conjugated phospholipids are substantially different from those of similar model systems but that the ANO2 antibodies, when bound, display similar diffusive behavior.
引用
收藏
页码:219 / 228
页数:10
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