SYNTHESIS OF OPTICALLY-ACTIVE CYANOHYDRINS USING R-OXYNITRILASE IN A LIQUID-LIQUID BIPHASIC SYSTEM .1. AN INDUSTRIALLY USEFUL PROCEDURE

被引：35

作者：

LOOS, WT ^{[1
]}

GELUK, HW ^{[1
]}

RUIJKEN, MMA ^{[1
]}

KRUSE, CG ^{[1
]}

BRUSSEE, J ^{[1
]}

VANDERGEN, A ^{[1
]}

机构：

[1] LEIDEN UNIV,LEIDEN INST CHEM,GORLAEUS LABS,2300 RA LEIDEN,NETHERLANDS

来源：

BIOCATALYSIS AND BIOTRANSFORMATION | 1995年 / 12卷 / 04期

关键词：

R-OXYNITRILASE; R-CYANOHYDRINS; EFFECTS OF REACTION PARAMETERS; ENZYME STABILITY;

D O I：

10.3109/10242429509003188

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

An improved method is presented for preparing optically active cyanohydrins in high yield and high enantioselectivity, using R-oxynitrilase from almonds as the catalyst in a biphasic water-organic solvent system. Reaction conditions for two representative substates have been studied with respect to conversion, optical purity and enzyme stability. The suitability of the procedure for industrial use was substantiated by the possibility of reusing the enzyme-containing aqueous phase for the synthesis of R-mandelonitrile.

引用

页码：255 / 266

页数：12

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