REGULATION OF HUMAN GROWTH-HORMONE RECEPTOR GENE-EXPRESSION BY HUMAN GROWTH-HORMONE IN A HUMAN HEPATOMA-CELL LINE

被引:39
作者
MULLIS, PE
LUND, T
PATEL, MS
BROOK, CGD
BRICKELL, PM
机构
[1] UNIV COLL & MIDDLESEX SCH MED, DEPT BIOCHEM, MED MOLEC BIOL UNIT, LONDON, ENGLAND
[2] UNIV COLL & MIDDLESEX SCH MED, MIDDLESEX HOSP, COBBOLD LABS, ENDOCRINE UNIT, LONDON, ENGLAND
[3] UNIV COLL & MIDDLESEX SCH MED, DEPT IMMUNOL, LONDON, ENGLAND
关键词
GROWTH HORMONE; GROWTH HORMONE RECEPTOR; TRANSCRIPTION; HEPATOMA CELL LINE;
D O I
10.1016/0303-7207(91)90267-V
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
We have investigated the effects of recombinant human growth hormone (r-hGH) on the expression of hGH-receptor in a human hepatoma cell line (HuH 7). Levels of hGH-receptor mRNA in HuH 7 cells treated with different doses of r-hGH were measured by means of an RNase protection assay. Treatment with r-hGH at physiological concentrations (12.5, 25 and 50 ng/ml) resulted in an increase in hGH-receptor mRNA levels within 1 h of addition of the hormone. A steady state was reached after 3-4 h and maintained for at least 48 h. In contrast, treatment with supraphysiological r-hGH concentrations (150 and 500 ng/ml) led to a down-regulation of hGH-receptor mRNA levels during the first 3 h after hormone addition followed by an increase in hGH-receptor mRNA levels thereafter. Nuclear run-off assays demonstrated that these changes in hGH-receptor mRNA levels were a result of changes in the rate of transcription of the hGH-receptor gene. Cycloheximide (10-mu-g/ml) did not affect these changes in hGH-receptor gene transcription significantly, indicating that they are mediated by pre-existing factors and do not require new protein synthesis. These data demonstrate that r-hGH specifically regulates the rate of transcription of the hGH-receptor gene in a human hepatoma cell line.
引用
收藏
页码:125 / 133
页数:9
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