USE OF 2 OXONOLS AND A FLUORESCENT TETRAZOLIUM DYE TO MONITOR STARVATION OF ESCHERICHIA-COLI IN SEAWATER BY FLOW-CYTOMETRY

被引：43

作者：

LOPEZAMOROS, R ^{[1
]}

MASON, DJ ^{[1
]}

LLOYD, D ^{[1
]}

机构：

[1] UNIV WALES COLL CARDIFF,SCH PURE & APPL BIOL,MICROBIOL GRP,CARDIFF CF1 3TL,S GLAM,WALES

来源：

JOURNAL OF MICROBIOLOGICAL METHODS | 1995年 / 22卷 / 02期

关键词：

ESCHERICHIA-COLI; SEAWATER; MEMBRANE POTENTIAL DYE; REDOX DYE; FLOW CYTOMETRY; STARVATION;

D O I：

10.1016/0167-7012(94)00073-G

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Flow cytometry was used to study starvation of Escherichia coli in artificial seawater using two membrane potential sensitive dyes and redox dye. The oxonols, DiBAC4(3) and oxonol VI, enabled assessment of energization states. Parallel use of these two dyes suggests that oxonol VI is the more useful for detection of membrane depolarization. The NADPH reducibility of 5-cyano-2,3-ditolyl tetrazolium chloride can be used to estimate loss of membrane integrity during survival in seawater.

引用

页码：165 / 176

页数：12

共 24 条

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