GENETIC-ANALYSIS OF SUPRAOPERONIC CLUSTERING BY USE OF NATURAL TRANSFORMATION IN ACINETOBACTER-CALCOACETICUS

被引：30

作者：

AVERHOFF, B ^{[1
]}

GREGGJOLLY, L ^{[1
]}

ELSEMORE, D ^{[1
]}

ORNSTON, LN ^{[1
]}

机构：

[1] YALE UNIV,DEPT BIOL,NEW HAVEN,CT 06511

来源：

JOURNAL OF BACTERIOLOGY | 1992年 / 174卷 / 01期

关键词：

D O I：

10.1128/jb.174.1.200-204.1992

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

DNA within Escherichia coli colonies carrying cloned Acinetobacter calcoaceticus genes transforms mutant A. calcoaceticus cells with high efficiency. Therefore, E. coli colonies containing such cloned genes can be identified by replica plating onto a lawn of A. calcoaceticus mutant cells. Transformation of A. calcoaceticus also facilitates gap repair and thus allows recovery of specified chromosomal segments in recombinant plasmids. These procedures were used to demonstrate the clustering of A. calcoaceticus genes required for utilization of p-hydroxybenzoate. Chromosomal linkage of the bacterial genes, contained in different operons separated by about 10 kbp of DNA, may have been selected on the basis of their physiological interdependence.

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页码：200 / 204

页数：5

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