Fluorescence in situ hybridization provides evidence for two-step rearrangement in a masked Ph chromosome formation

被引：12

作者：

Sessarego, M ^{[1
]}

Fugazza, G ^{[1
]}

Canepa, L ^{[1
]}

Bacigalupo, A ^{[1
]}

Bruzzone, R ^{[1
]}

Patrone, F ^{[1
]}

机构：

[1] OSPED SAN MARTINO GENOVA,DIV EMATOL 2,GENOA,ITALY

来源：

LEUKEMIA RESEARCH | 1995年 / 19卷 / 12期

关键词：

CML; masked Ph; FISH;

D O I：

10.1016/0145-2126(95)00093-3

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

A chronic myelogenous leukemia (CML) patient with a masked Ph chromosome due to the translocation (9;10;22)(q34;q24;q11) is reported. Banding analysis showed a 9q+ chromosome typical of standard t(9;22)(q34;q11), and fluorescence in situ hybridization studies confirmed the involvement of a chromosome 10 in the masked Ph formation and also the presence of 3' ABL-DNA sequences in the der(22). This complex rearrangement could be explained by two consecutive translocations: the first, a standard t(9;22) (q34;q11), the second, a translocation between a chromosome 10 and the der(22) with a breakpoint in sequences derived from chromosome 9 telomeric to the ABL gene. By reverse transcription polymerase chain reaction (RT-PCR), we studied the BCR/ABL transcript junction: a chimeric m-RNA b3-a2, indicating a breakpoint within the major breakpoint cluster region, was found.

引用

页码：921 / 925

页数：5

共 19 条

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