HISTOCHEMICAL-DEMONSTRATION OF CALCIUM ACCUMULATION IN MUSCLE-FIBERS AFTER EXPERIMENTAL ORGANOPHOSPHATE POISONING

被引：24

作者：

INNS, RH

TUCKWELL, NJ

BRIGHT, JE

MARRS, TC

机构：

[1] CDE Porton Down, Salisbury

来源：

HUMAN & EXPERIMENTAL TOXICOLOGY | 1990年 / 9卷 / 04期

关键词：

D O I：

10.1177/096032719000900407

中图分类号：

R99 [毒物学（毒理学）];

学科分类号：

100405 ;

摘要：

The LD50 of subcutaneously-injected sarin (GB: isopropyl methylphosphonofluoridate) in mice was 172 μg kg-1. Mice were treated with sarin at doses between 25 and 150 μg kg-1, administered subcutaneously. After sacrifice of the animals, the diaphragms were removed and stained for acetylcholinesterase activity and the presence of ionized calcium. Calcium was found in the diaphragms of those mice to which sarin had been administered at doses of 50 μg kg-1 or above. Calcium accumulation was not present in diaphragms from those animals that had received 25 μg kg-1. Calcium accumulation occurred earliest and remained longest in diaphragms from those animals receiving the highest doses. Accumulation of calcium was associated with end-plates, as demonstrated by an acetylcholinesterase histochemical method. © 1990, Sage Publications. All rights reserved.

引用

页码：245 / 250

页数：6

共 19 条

[11] APPLICABILITY OF A NEW SCHIFF BASE, GLYOXAL BIS (2-HYDROXY-ANIL), FOR CYTOCHEMICAL LOCALIZATION OF IONIC CALCIUM [J].