CRYSTAL-STRUCTURE OF D(CGCGAATTCGCG) COMPLEXED WITH PROPAMIDINE, A SHORT-CHAIN HOMOLOG OF THE DRUG PENTAMIDINE

被引：67

作者：

NUNN, CM ^{[1
]}

JENKINS, TC ^{[1
]}

NEIDLE, S ^{[1
]}

机构：

[1] INST CANC RES,CANC RES CAMPAIGN,BIOMOLEC STRUCT UNIT,SUTTON SM2 5NG,SURREY,ENGLAND

来源：

BIOCHEMISTRY | 1993年 / 32卷 / 50期

关键词：

D O I：

10.1021/bi00213a012

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The crystal structure of the complex between propamidine and the self-complementary DNA dodecamer d(CGCGAATTCGCG) duplex has been determined to a resolution of 2.1 angstrom and an R-factor of 17.4%. The propamidine binds to the DNA within the minor groove, with specific hydrogen-bonding interactions from the amidinium groups to the bases and sugar groups of the DNA, via a 4-base-pair binding site. A network of water molecules lies over the mouth of the minor groove. The structure provides a rationale for the superior DNA-binding properties of propamidine as compared to pentamidine [cf. Edwards, K. J., Jenkins, T. C., & Neidle, S. (1992) Biochemistry 31, 7104-7109]. This is seen to result from (i) decreased minor groove width at the binding site and (ii) reduced mobility of the bound propamidine molecule.

引用

页码：13838 / 13843

页数：6

共 27 条

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