CRYSTAL-STRUCTURE OF D(CGCGAATTCGCG) COMPLEXED WITH PROPAMIDINE, A SHORT-CHAIN HOMOLOG OF THE DRUG PENTAMIDINE

被引：67

作者：

NUNN, CM ^{[1
]}

JENKINS, TC ^{[1
]}

NEIDLE, S ^{[1
]}

机构：

[1] INST CANC RES,CANC RES CAMPAIGN,BIOMOLEC STRUCT UNIT,SUTTON SM2 5NG,SURREY,ENGLAND

来源：

BIOCHEMISTRY | 1993年 / 32卷 / 50期

关键词：

D O I：

10.1021/bi00213a012

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The crystal structure of the complex between propamidine and the self-complementary DNA dodecamer d(CGCGAATTCGCG) duplex has been determined to a resolution of 2.1 angstrom and an R-factor of 17.4%. The propamidine binds to the DNA within the minor groove, with specific hydrogen-bonding interactions from the amidinium groups to the bases and sugar groups of the DNA, via a 4-base-pair binding site. A network of water molecules lies over the mouth of the minor groove. The structure provides a rationale for the superior DNA-binding properties of propamidine as compared to pentamidine [cf. Edwards, K. J., Jenkins, T. C., & Neidle, S. (1992) Biochemistry 31, 7104-7109]. This is seen to result from (i) decreased minor groove width at the binding site and (ii) reduced mobility of the bound propamidine molecule.

引用

页码：13838 / 13843

页数：6

共 27 条

[21] CRYSTAL-STRUCTURE ANALYSIS OF THE B-DNA DODECAMER CGTGAATTCACG
LARSEN, TA
KOPKA, ML
DICKERSON, RE
[J]. BIOCHEMISTRY, 1991, 30 (18) : 4443 - 4449
[22] MONTGOMERY AB, 1987, LANCET, V2, P480
[23] HYDRATION OF DNA BASES - ANALYSIS OF CRYSTALLOGRAPHIC DATA
SCHNEIDER, B
COHEN, D
BERMAN, HM
[J]. BIOPOLYMERS, 1992, 32 (07) : 725 - 750
[24] Sussman J. L., 1984, Methods and Applications in Crystallographic Computing. International Summer School on Crystallographic Computing, P206
[25] ANALOGS OF 1,5-BIS(4-AMIDINOPHENOXY)PENTANE (PENTAMIDINE) IN THE TREATMENT OF EXPERIMENTAL PNEUMOCYSTIS-CARINII PNEUMONIA
TIDWELL, RR
JONES, SK
GERATZ, JD
OHEMENG, KA
CORY, M
HALL, JE
[J]. JOURNAL OF MEDICINAL CHEMISTRY, 1990, 33 (04) : 1252 - 1257
[26] WISPELWEY B, 1991, INFECT CONT HOSP EP, V12, P375, DOI 10.1086/646360
[27] STRUCTURE OF AN ALTERNATING-B DNA HELIX AND ITS RELATIONSHIP TO A-TRACT DNA
YOON, C
PRIVE, GG
GOODSELL, DS
DICKERSON, RE
[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1988, 85 (17) : 6332 - 6336

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